NLRP3 in somatic non-immune cells of rodent and primate testes

Lena Walenta, Nina Schmid, J. Ullrich Schwarzer, Frank Michael Köhn, Henryk Urbanski, Rüdiger Behr, Leena Strauss, Matti Poutanen, Artur Mayerhofer

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    NLRP3 is part of the NLRP3 inflammasome and a global sensor of cellular damage. It was recently discovered in rodent Sertoli cells. We investigated NLRP3 in mouse, human and non-human primate (marmoset and rhesus macaque) testes, employing immunohistochemistry. Sertoli cells of all species expressed NLRP3, and the expression preceded puberty. In addition, peritubular cells of the adult human testes expressed NLRP3. NLRP3 and associated genes (PYCARD, CASP1, IL1B) were also found in isolated human testicular peritubular cells and the mouse Sertoli cell line TM4. Male infertility due to impairments of spermatogenesis may be related to sterile inflammatory events. We observed that the expression of NLRP3 was altered in the testes of patients suffering from mixed atrophy syndrome, in which tubules with impairments of spermatogenesis showed prominent NLRP3 staining. In order to explore a possible role of NLRP3 in male infertility, associated with sterile testicular inflammation, we studied a mouse model of male infertility. These human aromatase-expressing transgenic mice (AROM+) develop testicular inflammation and impaired spermatogenesis during aging, and the present data show that this is associated with strikingly elevated Nlrp3 expression in the testes compared to WT controls. Interference by aromatase inhibitor treatment significantly reduced increased Nlrp3 levels. Thus, throughout species NLRP3 is expressed by somatic cells of the testis, which are involved in testicular immune surveillance. We conclude that NLRP3 may be a novel player in testicular immune regulation.

    Original languageEnglish (US)
    Pages (from-to)231-238
    Number of pages8
    JournalReproduction
    Volume156
    Issue number3
    DOIs
    StatePublished - Jan 1 2018

    Fingerprint

    Primates
    Testis
    Rodentia
    Sertoli Cells
    Male Infertility
    Spermatogenesis
    Inflammasomes
    Inflammation
    Callithrix
    Aromatase Inhibitors
    Aromatase
    Puberty
    Macaca mulatta
    Transgenic Mice
    Atrophy
    Immunohistochemistry
    Staining and Labeling
    Cell Line
    Genes
    Therapeutics

    ASJC Scopus subject areas

    • Reproductive Medicine
    • Embryology
    • Endocrinology
    • Obstetrics and Gynecology
    • Cell Biology

    Cite this

    Walenta, L., Schmid, N., Ullrich Schwarzer, J., Köhn, F. M., Urbanski, H., Behr, R., ... Mayerhofer, A. (2018). NLRP3 in somatic non-immune cells of rodent and primate testes. Reproduction, 156(3), 231-238. https://doi.org/10.1530/REP-18-0111

    NLRP3 in somatic non-immune cells of rodent and primate testes. / Walenta, Lena; Schmid, Nina; Ullrich Schwarzer, J.; Köhn, Frank Michael; Urbanski, Henryk; Behr, Rüdiger; Strauss, Leena; Poutanen, Matti; Mayerhofer, Artur.

    In: Reproduction, Vol. 156, No. 3, 01.01.2018, p. 231-238.

    Research output: Contribution to journalArticle

    Walenta, L, Schmid, N, Ullrich Schwarzer, J, Köhn, FM, Urbanski, H, Behr, R, Strauss, L, Poutanen, M & Mayerhofer, A 2018, 'NLRP3 in somatic non-immune cells of rodent and primate testes', Reproduction, vol. 156, no. 3, pp. 231-238. https://doi.org/10.1530/REP-18-0111
    Walenta L, Schmid N, Ullrich Schwarzer J, Köhn FM, Urbanski H, Behr R et al. NLRP3 in somatic non-immune cells of rodent and primate testes. Reproduction. 2018 Jan 1;156(3):231-238. https://doi.org/10.1530/REP-18-0111
    Walenta, Lena ; Schmid, Nina ; Ullrich Schwarzer, J. ; Köhn, Frank Michael ; Urbanski, Henryk ; Behr, Rüdiger ; Strauss, Leena ; Poutanen, Matti ; Mayerhofer, Artur. / NLRP3 in somatic non-immune cells of rodent and primate testes. In: Reproduction. 2018 ; Vol. 156, No. 3. pp. 231-238.
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