Nitrogen and oxygen regulation of Bacillus subtilis nasDEF encoding NADH-dependent nitrite reductase by TnrA and ResDE

Michiko Nakano, Tamara Hoffmann, Yi Zhu, Dieter Jahn

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

The nitrate and nitrite reductases of Bacillus subtilis have two different physiological functions. Under conditions of nitrogen limitation, these enzymes catalyze the reduction of nitrate via nitrite to ammonia for the anabolic incorporation of nitrogen into biomolecules. They also function catabolically in anaerobic respiration, which involves the use of nitrate and nitrite as terminal electron acceptors. Two distinct nitrate reductases, encoded by narGHI and nusBC, function in anabolic and catabolic nitrogen metabolism, respectively. However, as reported herein, a single NADH- dependent, soluble nitrite reductase encoded by the nasDE genes is required for both catabolic and anabolic processes. The nasDE genes, together with nasBC (encoding assimilatory nitrate reductase) and nasF (required for nitrite reductase siroheme cofactor formation), constitute the has operon. Data presented show that transcription of nasDEF is driven not only by the previously characterized nus operon promoter but also from an internal promoter residing between the nasC and nasd genes. Transcription from both promoters is activated by nitrogen limitation during aerobic growth by the nitrogen regulator, TnrA. However, under conditions of oxygen limitation, nasDEF expression and nitrite reductase activity were significantly induced. Anaerobic induction of nasDEF required the ResDE two-component regulatory system and the presence of nitrite, indicating partial coregulation of NasDEF with the respiratory nitrate reductase NarGHI during nitrate respiration.

Original languageEnglish (US)
Pages (from-to)5344-5350
Number of pages7
JournalJournal of Bacteriology
Volume180
Issue number20
StatePublished - Oct 1998

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Nitrite Reductase (NAD(P)H)
Bacillus subtilis
Nitrites
Nitrogen
Oxygen
Nitrate Reductases
Nitrates
Nitrite Reductases
Nitrate Reductase
Operon
Respiration
Genes
Ammonia
Electrons
Enzymes
Growth

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Nitrogen and oxygen regulation of Bacillus subtilis nasDEF encoding NADH-dependent nitrite reductase by TnrA and ResDE. / Nakano, Michiko; Hoffmann, Tamara; Zhu, Yi; Jahn, Dieter.

In: Journal of Bacteriology, Vol. 180, No. 20, 10.1998, p. 5344-5350.

Research output: Contribution to journalArticle

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T1 - Nitrogen and oxygen regulation of Bacillus subtilis nasDEF encoding NADH-dependent nitrite reductase by TnrA and ResDE

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