Nitrogen-13-labeled nitrite and nitrate: Distribution and metabolism after intratracheal administration

Norris J. Parks; Kenneth A. Krohn; Chester A. Mathis; Joseph H. Chasko; Kenneth R. Geiger; Marsha E. Gregor; Neal F. Peek

doi:10.1126/science.7209517

Nitrogen-13-labeled nitrite and nitrate: Distribution and metabolism after intratracheal administration

Norris J. Parks, Kenneth A. Krohn, Chester A. Mathis, Joseph H. Chasko, Kenneth R. Geiger, Marsha E. Gregor, Neal F. Peek

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Abstract

Radioactive nitrogen-13 from nitrite (NO₂^-) or nitrate (NO₃^-) administered intratracheally or intravenously without added carrier to mice or rabbits was distributed evenly throughout most organs and tissues regardless of the entry route or the anion administered. Nitrogen-13 from both anions was distributed uniformly between plasma and blood cells. We found rapid in vivo oxidation of NO₂ ^- to NO₃^- at concentrations of 2 to 3 nanomoles per liter in blood. Over 50 percent oxidation within 10 minutes accounted for the similar nitrogen-13 distributions from both parent ions. The oxidation rates were animal species-dependent. No reduction of ¹³NO₃ ^- to ¹³NO₂^- was observed. A mechanistic hypothesis invoking oxidation of ¹³NO₂ ^- by a catalase-hydrogen peroxide complex accounts for the results. These results imply a concentration dependence for the in vivo fate of NO ₂^- or nitrogen dioxide.

Original language	English (US)
Pages (from-to)	58-61
Number of pages	4
Journal	Science
Volume	212
Issue number	4490
DOIs	https://doi.org/10.1126/science.7209517
State	Published - 1981
Externally published	Yes

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@article{5d41fafe4836460d81ad9895f6e99cc3,

title = "Nitrogen-13-labeled nitrite and nitrate: Distribution and metabolism after intratracheal administration",

abstract = "Radioactive nitrogen-13 from nitrite (NO2-) or nitrate (NO3-) administered intratracheally or intravenously without added carrier to mice or rabbits was distributed evenly throughout most organs and tissues regardless of the entry route or the anion administered. Nitrogen-13 from both anions was distributed uniformly between plasma and blood cells. We found rapid in vivo oxidation of NO2 - to NO3- at concentrations of 2 to 3 nanomoles per liter in blood. Over 50 percent oxidation within 10 minutes accounted for the similar nitrogen-13 distributions from both parent ions. The oxidation rates were animal species-dependent. No reduction of 13NO3 - to 13NO2- was observed. A mechanistic hypothesis invoking oxidation of 13NO2 - by a catalase-hydrogen peroxide complex accounts for the results. These results imply a concentration dependence for the in vivo fate of NO 2- or nitrogen dioxide.",

author = "Parks, {Norris J.} and Krohn, {Kenneth A.} and Mathis, {Chester A.} and Chasko, {Joseph H.} and Geiger, {Kenneth R.} and Gregor, {Marsha E.} and Peek, {Neal F.}",

year = "1981",

doi = "10.1126/science.7209517",

language = "English (US)",

volume = "212",

pages = "58--61",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "4490",

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TY - JOUR

T1 - Nitrogen-13-labeled nitrite and nitrate

T2 - Distribution and metabolism after intratracheal administration

AU - Parks, Norris J.

AU - Krohn, Kenneth A.

AU - Mathis, Chester A.

AU - Chasko, Joseph H.

AU - Geiger, Kenneth R.

AU - Gregor, Marsha E.

AU - Peek, Neal F.

PY - 1981

Y1 - 1981

N2 - Radioactive nitrogen-13 from nitrite (NO2-) or nitrate (NO3-) administered intratracheally or intravenously without added carrier to mice or rabbits was distributed evenly throughout most organs and tissues regardless of the entry route or the anion administered. Nitrogen-13 from both anions was distributed uniformly between plasma and blood cells. We found rapid in vivo oxidation of NO2 - to NO3- at concentrations of 2 to 3 nanomoles per liter in blood. Over 50 percent oxidation within 10 minutes accounted for the similar nitrogen-13 distributions from both parent ions. The oxidation rates were animal species-dependent. No reduction of 13NO3 - to 13NO2- was observed. A mechanistic hypothesis invoking oxidation of 13NO2 - by a catalase-hydrogen peroxide complex accounts for the results. These results imply a concentration dependence for the in vivo fate of NO 2- or nitrogen dioxide.

AB - Radioactive nitrogen-13 from nitrite (NO2-) or nitrate (NO3-) administered intratracheally or intravenously without added carrier to mice or rabbits was distributed evenly throughout most organs and tissues regardless of the entry route or the anion administered. Nitrogen-13 from both anions was distributed uniformly between plasma and blood cells. We found rapid in vivo oxidation of NO2 - to NO3- at concentrations of 2 to 3 nanomoles per liter in blood. Over 50 percent oxidation within 10 minutes accounted for the similar nitrogen-13 distributions from both parent ions. The oxidation rates were animal species-dependent. No reduction of 13NO3 - to 13NO2- was observed. A mechanistic hypothesis invoking oxidation of 13NO2 - by a catalase-hydrogen peroxide complex accounts for the results. These results imply a concentration dependence for the in vivo fate of NO 2- or nitrogen dioxide.

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Nitrogen-13-labeled nitrite and nitrate: Distribution and metabolism after intratracheal administration

Abstract

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