Nitrogen-13-labeled nitrite and nitrate: Distribution and metabolism after intratracheal administration

Norris J. Parks, Kenneth Krohn, Chester A. Mathis, Joseph H. Chasko, Kenneth R. Geiger, Marsha E. Gregor, Neal F. Peek

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Radioactive nitrogen-13 from nitrite (NO2-) or nitrate (NO3-) administered intratracheally or intravenously without added carrier to mice or rabbits was distributed evenly throughout most organs and tissues regardless of the entry route or the anion administered. Nitrogen-13 from both anions was distributed uniformly between plasma and blood cells. We found rapid in vivo oxidation of NO2 - to NO3- at concentrations of 2 to 3 nanomoles per liter in blood. Over 50 percent oxidation within 10 minutes accounted for the similar nitrogen-13 distributions from both parent ions. The oxidation rates were animal species-dependent. No reduction of 13NO3 - to 13NO2- was observed. A mechanistic hypothesis invoking oxidation of 13NO2 - by a catalase-hydrogen peroxide complex accounts for the results. These results imply a concentration dependence for the in vivo fate of NO 2- or nitrogen dioxide.

Original languageEnglish (US)
Pages (from-to)58-61
Number of pages4
JournalScience
Volume212
Issue number4490
StatePublished - 1981
Externally publishedYes

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Nitrites
Nitrates
Nitrogen
Anions
Nitrogen Dioxide
Plasma Cells
Catalase
Hydrogen Peroxide
Blood Cells
Ions
Rabbits

ASJC Scopus subject areas

  • General

Cite this

Parks, N. J., Krohn, K., Mathis, C. A., Chasko, J. H., Geiger, K. R., Gregor, M. E., & Peek, N. F. (1981). Nitrogen-13-labeled nitrite and nitrate: Distribution and metabolism after intratracheal administration. Science, 212(4490), 58-61.

Nitrogen-13-labeled nitrite and nitrate : Distribution and metabolism after intratracheal administration. / Parks, Norris J.; Krohn, Kenneth; Mathis, Chester A.; Chasko, Joseph H.; Geiger, Kenneth R.; Gregor, Marsha E.; Peek, Neal F.

In: Science, Vol. 212, No. 4490, 1981, p. 58-61.

Research output: Contribution to journalArticle

Parks, NJ, Krohn, K, Mathis, CA, Chasko, JH, Geiger, KR, Gregor, ME & Peek, NF 1981, 'Nitrogen-13-labeled nitrite and nitrate: Distribution and metabolism after intratracheal administration', Science, vol. 212, no. 4490, pp. 58-61.
Parks NJ, Krohn K, Mathis CA, Chasko JH, Geiger KR, Gregor ME et al. Nitrogen-13-labeled nitrite and nitrate: Distribution and metabolism after intratracheal administration. Science. 1981;212(4490):58-61.
Parks, Norris J. ; Krohn, Kenneth ; Mathis, Chester A. ; Chasko, Joseph H. ; Geiger, Kenneth R. ; Gregor, Marsha E. ; Peek, Neal F. / Nitrogen-13-labeled nitrite and nitrate : Distribution and metabolism after intratracheal administration. In: Science. 1981 ; Vol. 212, No. 4490. pp. 58-61.
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AU - Parks, Norris J.

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AU - Geiger, Kenneth R.

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AU - Peek, Neal F.

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