Nitrogen-13-labeled nitrite and nitrate: Distribution and metabolism after intratracheal administration

Norris J. Parks, Kenneth Krohn, Chester A. Mathis, Joseph H. Chasko, Kenneth R. Geiger, Marsha E. Gregor, Neal F. Peek

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Abstract

Radioactive nitrogen-13 from nitrite (NO2-) or nitrate (NO3-) administered intratracheally or intravenously without added carrier to mice or rabbits was distributed evenly throughout most organs and tissues regardless of the entry route or the anion administered. Nitrogen-13 from both anions was distributed uniformly between plasma and blood cells. We found rapid in vivo oxidation of NO2 - to NO3- at concentrations of 2 to 3 nanomoles per liter in blood. Over 50 percent oxidation within 10 minutes accounted for the similar nitrogen-13 distributions from both parent ions. The oxidation rates were animal species-dependent. No reduction of 13NO3 - to 13NO2- was observed. A mechanistic hypothesis invoking oxidation of 13NO2 - by a catalase-hydrogen peroxide complex accounts for the results. These results imply a concentration dependence for the in vivo fate of NO 2- or nitrogen dioxide.

Original languageEnglish (US)
Pages (from-to)58-61
Number of pages4
JournalScience
Volume212
Issue number4490
Publication statusPublished - 1981
Externally publishedYes

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Cite this

Parks, N. J., Krohn, K., Mathis, C. A., Chasko, J. H., Geiger, K. R., Gregor, M. E., & Peek, N. F. (1981). Nitrogen-13-labeled nitrite and nitrate: Distribution and metabolism after intratracheal administration. Science, 212(4490), 58-61.