Nitric oxide synthase inhibition attenuates hypoglycemic cerebral hyperemia in piglets

R. N. Ichord, M. A. Helfaer, Jeffrey Kirsch, D. Wilson, R. J. Traystman

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We tested the hypothesis that nitric oxide (NO) mediates hypoglycemia- induced cerebral vasodilation in piglets. Piglets (1-2 wk old) were made hypoglycemic with insulin (200 U/kg iv) with and without an NO synthase inhibitor, N(ω)-nitro-L-arginine methyl ester (L-NAME, 40 mg/kg iv). Electroencephalogram (EEG), cerebral O2 consumption (CMR(O2)), and cerebral blood flow (CBF) were measured before L-NAME and insulin and for 180 min after insulin. Hypoglycemia led to isoelectric EEG earlier after L-NAME (87 ± 8 min) than without L-NAME pretreatment (132 ± 13 min). CBF increased in all brain regions during hypoglycemia at the onset of isoelectric EEG and was associated with increased CMR(O2). L-NAME prevented the increase in CMR(O2) and attenuated vasodilation in forebrain (154 ± 37 vs. 400 ± 60%), cerebellum (251 ± 52 vs. 386 ± 52%), and cortical gray matter (183 ± 47 vs. 524 ± 93%) but had no effect on CBF responses in brain stem, thalamus, caudate, or hippocampus. We conclude that NO or a NO-containing compound mediates cerebral vasodilation induced by profound insulin-hypoglycemia in piglets and that this vasodilation plays an important role in the adaptation of immature brain to hypoglycemia.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume266
Issue number3 35-3
StatePublished - 1994
Externally publishedYes

Fingerprint

hypoglycemia
NG-Nitroarginine Methyl Ester
Hyperemia
nitric oxide synthase
Cerebrovascular Circulation
Hypoglycemia
Hypoglycemic Agents
Nitric Oxide Synthase
vasodilation
piglets
Vasodilation
electroencephalography
insulin
Insulin
blood flow
nitric oxide
Electroencephalography
Nitric Oxide
brain
thalamus

Keywords

  • cerebral blood flow
  • electroencephalogram
  • hypoglycemia
  • infant
  • N(ω)-nitro-L-arginine methyl ester

ASJC Scopus subject areas

  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Nitric oxide synthase inhibition attenuates hypoglycemic cerebral hyperemia in piglets. / Ichord, R. N.; Helfaer, M. A.; Kirsch, Jeffrey; Wilson, D.; Traystman, R. J.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 266, No. 3 35-3, 1994.

Research output: Contribution to journalArticle

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