Nitric oxide-sensitive and -insensitive interaction of Bacillus subtilis NsrR with a ResDE-controlled promoter

Sushma Kommineni, Erik Yukl, Takahiro Hayashi, Jacob Delepine, Hao Geng, Pierre Moenne-Loccoz, Michiko Nakano

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

NsrR is a nitric oxide (NO)-sensitive transcription repressor that controls NO metabolism in a wide range of bacteria. In Bacillus subtilis, NsrR represses transcription of the nitrite reductase (nasDEF) genes that are under positive control of the ResD-ResE two-component signal transduction system. Derepression is achieved by reaction of NO with NsrR. Unlike some NsrR orthologues that were shown to contain a NO-sensitive [2Fe-2S] cluster, B. subtilis NsrR, when purified anaerobically either from aerobic or from anaerobic Escherichia coli and B. subtilis cultures, contains a [4Fe-4S] cluster. [4Fe-4S]-NsrR binds around the -35 element of the nasD promoter with much higher affinity than apo-NsrR and binding of [4Fe-4S]-NsrR, but not apo-protein, is sensitive to NO. RNA polymerase and phosphorylated ResD make a ternary complex at the nasD promoter and NsrR dissociates the preformed ternary complex. In addition to the -35 region, NsrR binds to two distinct sites of the upstream regulatory region where ResD also binds. These interactions, unlike the high-affinity site binding, do not depend on the NsrR [4Fe-4S] cluster and binding is not sensitive to NO, suggesting a role for apo-NsrR in transcriptional regulation.

Original languageEnglish (US)
Pages (from-to)1280-1293
Number of pages14
JournalMolecular Microbiology
Volume78
Issue number5
DOIs
StatePublished - Dec 2010

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Bacillus subtilis
Nitric Oxide
Nitrite Reductases
Nucleic Acid Regulatory Sequences
DNA-Directed RNA Polymerases
Signal Transduction
Binding Sites
Escherichia coli
Bacteria
Genes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Nitric oxide-sensitive and -insensitive interaction of Bacillus subtilis NsrR with a ResDE-controlled promoter. / Kommineni, Sushma; Yukl, Erik; Hayashi, Takahiro; Delepine, Jacob; Geng, Hao; Moenne-Loccoz, Pierre; Nakano, Michiko.

In: Molecular Microbiology, Vol. 78, No. 5, 12.2010, p. 1280-1293.

Research output: Contribution to journalArticle

Kommineni, Sushma ; Yukl, Erik ; Hayashi, Takahiro ; Delepine, Jacob ; Geng, Hao ; Moenne-Loccoz, Pierre ; Nakano, Michiko. / Nitric oxide-sensitive and -insensitive interaction of Bacillus subtilis NsrR with a ResDE-controlled promoter. In: Molecular Microbiology. 2010 ; Vol. 78, No. 5. pp. 1280-1293.
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