Nitric oxide and mitochondrial status in noise-induced hearing loss

Xiaorui Shi, Weiju Han, Hiroshi Yamamoto, Irina Omelchenko, Alfred Nuttall

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

This study investigated the distribution of nitric oxide (NO) within isolated outer hair cells (OHCs) from the cochlea, its relationship to mitochondria and its modulation of mitochondrial function. Using two fluorescent dyes - 4,5-diaminofluorescein diacetate (DAF-2DA), which detects NO, and tetramethyl rhodamine methyl ester (TMRM+), a mitochondrial membrane potential dye - it was found that a relatively greater amount of the DAF fluorescence in OHCs co-localized with mitochondria in comparison to DAF fluorescence in the cytosole. This study also observed reduced mitochondrial membrane potential of OHCs and increased DAF fluorescence following exposure of the cells to noise (120 dB SPL for 4 h) and to an exogenous NO donor, NOC-7 (≥350 nm). Antibody label for nitrotyrosine was also increased, indicating NO-related formation of peroxynitrite in both mitochrondria and the cytosol. The results suggest that NO may play an important physiological role in regulating OHC energy status and act as a potential agent in OHC pathology.

Original languageEnglish (US)
Pages (from-to)1313-1325
Number of pages13
JournalFree Radical Research
Volume41
Issue number12
DOIs
StatePublished - Dec 1 2007

Keywords

  • Mitochondria
  • Nitric oxide
  • Noise exposure
  • Outer hair cells

ASJC Scopus subject areas

  • Biochemistry

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