The component in human transfer factor (TF) (Fraction IV, from exclusion chromatography on Sephadex G 25) responsible for suppression of antigen induced lymphocyte transformation was previously identified as nicotinamide. Commercially available nicotinamide was subsequently shown to produce suppression of antigen induced responses in vitro previously observed with TF Fraction IV. Nicotinamide was found to be nontoxic at the highest concentrations employed (10 -2 M) and suppressive over a relatively broad range (10 -5 to 10 -2 M). The suppression appeared to be related to the magnitude of antigen or mitogen induced transformation and was apparent even when nicotinamide was added as late as 48 hr after stimulant addition.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Immunology|
|Publication status||Published - 1976|
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