New recombinant vaccines based on the use of prokaryotic antigen-display system

Piergiuseppe De Berardinis, Nancy Haigwood

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A major challenge in vaccine design has been to identify antigen presentation systems that elicit strong T- and B-cell responses. In the authors' laboratory, two new delivery vehicles derived from nonpathogenic prokaryotic organisms were recently designed and investigated. Conserved antigenic determinants were inserted into the N-terminal region of the major pVIII coat protein of bacteriophage fd virions or on the surface of an icosahedral scaffold formed by the acyltransferase component (E2 protein) of the pyruvate dehydrogenase complex of Bacillus stearothermophilus. The data indicate that the antigenic determinant displayed by either fd virions or on the surface of the E2 lattice are accessible to the immune system, and are able to trigger a humoral response as well as a potent helper and cytolytic response in vitro and in vivo. These systems offer the potential for safe and inexpensive vaccines to elicit full-spectrum immune responses.

Original languageEnglish (US)
Pages (from-to)673-679
Number of pages7
JournalExpert Review of Vaccines
Volume3
Issue number6
DOIs
StatePublished - Dec 2004
Externally publishedYes

Fingerprint

Synthetic Vaccines
Virion
Epitopes
Vaccines
Dihydrolipoyllysine-Residue Acetyltransferase
Bacteriophage M13
Geobacillus stearothermophilus
Acyltransferases
Antigens
Antigen Presentation
Capsid Proteins
Immune System
B-Lymphocytes
Proteins
In Vitro Techniques

Keywords

  • Filamentous bacteriophage
  • Pyruvate dehydrogenase multienzyme complex

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

New recombinant vaccines based on the use of prokaryotic antigen-display system. / De Berardinis, Piergiuseppe; Haigwood, Nancy.

In: Expert Review of Vaccines, Vol. 3, No. 6, 12.2004, p. 673-679.

Research output: Contribution to journalArticle

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