New high conductance DEP devices for detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood

Michael (Mike) Heller, Avery Sonnenberg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The ability to rapidly detect cell free circulating (cfc) DNA nanoparticulate biomarkers, drug delivery nanoparticles and virus directly from blood is a major challenge for nanomedicine. Previously, we have developed high conductance dielectrophoretic (HC-DEP) microarray devices which allowed cfc-DNA, cellular nanoparticles (mitochondria, etc.), nanoparticles and virus to be rapidly isolated from undiluted whole blood. Now we have developed novel capillary tube and pipette tip DEP devices which can be used to isolate cfc-DNA directly from a few microliters of whole blood. An AC voltage from 50-150 volts is applied at frequencies of 5kHz-10kHz which causes cfc-DNA to become concentrated on the tip of the device. The material captured on the tip can then be transferred to a collection tube for further analysis (PCR, genotyping, sequencing). The new HC-DEP devices are being used to collect cfc-DNA from Chronic Lymphocytic Leukemia (CLL) whole blood patient samples. Blood sample to PCR is achieved in less than twenty minutes. HC-DEP sets the stage for new "seamless" sample to answer diagnostic systems which will allow a variety of important cancer and other disease biomarkers to be rapidly isolated and analyzed directly from blood and other clinical samples.

Original languageEnglish (US)
Title of host publicationTechnical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
Pages360-363
Number of pages4
StatePublished - Nov 23 2011
Externally publishedYes
EventNanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011 - Boston, MA, United States
Duration: Jun 13 2011Jun 16 2011

Publication series

NameTechnical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
Volume3

Conference

ConferenceNanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
CountryUnited States
CityBoston, MA
Period6/13/116/16/11

Fingerprint

Biomarkers
DNA
Blood
Nanoparticles
Viruses
Medical nanotechnology
Capillary tubes
Mitochondria
Microarrays
Drug delivery
1-(2-(dodecyloxy)ethyl)pyrrolidine hydrochloride
Electric potential

Keywords

  • Biomarkers
  • Blood
  • Cancer diagnostics
  • cfc-DNA
  • Dielectrophoresis (DEP)
  • DNA
  • Nanoparticles

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

Cite this

Heller, M. M., & Sonnenberg, A. (2011). New high conductance DEP devices for detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood. In Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011 (pp. 360-363). (Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011; Vol. 3).

New high conductance DEP devices for detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood. / Heller, Michael (Mike); Sonnenberg, Avery.

Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011. 2011. p. 360-363 (Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011; Vol. 3).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Heller, MM & Sonnenberg, A 2011, New high conductance DEP devices for detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood. in Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011. Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011, vol. 3, pp. 360-363, Nanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011, Boston, MA, United States, 6/13/11.
Heller MM, Sonnenberg A. New high conductance DEP devices for detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood. In Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011. 2011. p. 360-363. (Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011).
Heller, Michael (Mike) ; Sonnenberg, Avery. / New high conductance DEP devices for detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood. Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011. 2011. pp. 360-363 (Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011).
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