Neutrophil adhesion to 24-hour IL-1-stimulated endothelial cells under flow conditions

David A. Jones, C. Wayne Smith, Louis Picker, Larry V. McIntire

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In this study, we examine neutrophil adhesion under flow conditions to cultured HUVEC stimulated for 4 or 24 h with IL-1. Interactions are measured using videomicroscopy and a parallel-plate flow system which is capable of distinguishing primary adhesion and rolling from secondary (firm) adhesion. We find that after 24 h, E-selectin does not contribute to primary adhesion, in contrast to a significant contribution after 4 h. Endothelial cell P- selectin does not contribute at either time point. Blocking or removing L- selectin from the neutrophil surface decreases adhesion 50 to 70% at either time point, and neuraminidase treatment of neutrophils decreases adhesion by 85%. These results suggest that after a 24-h stimulation, primary adhesion depends on a distinct selectin-like interaction in which a HUVEC receptor binds carbohydrates on neutrophil glycoproteins, including L-selectin. We also find that secondary adhesion in this system can be inhibited 90% with Ab blockade of CD18/ICAM-1 interactions after a 24-h stimulation, and that a combination of IL-1 and IL-4 selectively down-regulates the pathway for primary adhesion at 24 h. These results demonstrate that neutrophils adhere using different receptor pathways following 4- and 24-h stimulations, and provide experimental data characterizing some properties of the receptors involved. One of the pathways that is evident at 24 h appears to be a novel selectin-like interaction.

Original languageEnglish (US)
Pages (from-to)858-863
Number of pages6
JournalJournal of Immunology
Volume157
Issue number2
StatePublished - Jul 15 1996
Externally publishedYes

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Interleukin-1
Neutrophils
Endothelial Cells
L-Selectin
Selectins
Video Microscopy
P-Selectin
E-Selectin
Neuraminidase
Intercellular Adhesion Molecule-1
Interleukin-4
Glycoproteins
Down-Regulation
Carbohydrates

ASJC Scopus subject areas

  • Immunology

Cite this

Neutrophil adhesion to 24-hour IL-1-stimulated endothelial cells under flow conditions. / Jones, David A.; Smith, C. Wayne; Picker, Louis; McIntire, Larry V.

In: Journal of Immunology, Vol. 157, No. 2, 15.07.1996, p. 858-863.

Research output: Contribution to journalArticle

Jones, David A. ; Smith, C. Wayne ; Picker, Louis ; McIntire, Larry V. / Neutrophil adhesion to 24-hour IL-1-stimulated endothelial cells under flow conditions. In: Journal of Immunology. 1996 ; Vol. 157, No. 2. pp. 858-863.
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