Abstract
In this study, we examine neutrophil adhesion under flow conditions to cultured HUVEC stimulated for 4 or 24 h with IL-1. Interactions are measured using videomicroscopy and a parallel-plate flow system which is capable of distinguishing primary adhesion and rolling from secondary (firm) adhesion. We find that after 24 h, E-selectin does not contribute to primary adhesion, in contrast to a significant contribution after 4 h. Endothelial cell P- selectin does not contribute at either time point. Blocking or removing L- selectin from the neutrophil surface decreases adhesion 50 to 70% at either time point, and neuraminidase treatment of neutrophils decreases adhesion by 85%. These results suggest that after a 24-h stimulation, primary adhesion depends on a distinct selectin-like interaction in which a HUVEC receptor binds carbohydrates on neutrophil glycoproteins, including L-selectin. We also find that secondary adhesion in this system can be inhibited 90% with Ab blockade of CD18/ICAM-1 interactions after a 24-h stimulation, and that a combination of IL-1 and IL-4 selectively down-regulates the pathway for primary adhesion at 24 h. These results demonstrate that neutrophils adhere using different receptor pathways following 4- and 24-h stimulations, and provide experimental data characterizing some properties of the receptors involved. One of the pathways that is evident at 24 h appears to be a novel selectin-like interaction.
Original language | English (US) |
---|---|
Pages (from-to) | 858-863 |
Number of pages | 6 |
Journal | Journal of Immunology |
Volume | 157 |
Issue number | 2 |
State | Published - Jul 15 1996 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology