Neutrophil activation monitored by flow cytometry: Stimulation by phorbol diester is an all-or-none event

Dean G. Hafeman, Harden M. Mcconnell, Joe Gray, Philip N. Dean

Research output: Contribution to journalArticle

23 Scopus citations


The population dynamics of single-cell stimulation was analyzed by monitoring autofluorescence by flow cytometry. Stimulation of the respiratory burst in human neutrophils by 12-O-tetradecanoyl phorbol-13-acetate (TPA) caused a decline in highly fluorescent cells (characteristic of resting neutrophils) and a corresponding increase in the number of weakly fluorescent cells (characteristic of activated neutrophils). Increasing concentrations of TPA caused increasing numbers of cells to shift from the highly fluorescent population to the weakly fluorescent population without the appearance of intermediate populations. Thus the neutrophil respiratory burst, a component of neutrophil cytotoxic response, is triggered in an all-or none fashion.

Original languageEnglish (US)
Pages (from-to)673-675
Number of pages3
Issue number4533
Publication statusPublished - 1982
Externally publishedYes


ASJC Scopus subject areas

  • General

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