Neutropenia in three patients with rheumatic disorders. Suppression of granulopoiesis by cortisol-sensitive thymus-dependent lymphocytes

G. C. Bagby, J. D. Gabourel

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    Abstract

    A man with polymyalgia rheumatica (patient 1) and two patients (2 and 3) with Felty's syndrome had neutropenia at the time of diagnosis. Bone marrow samples in each patient were cellular but showed an 'arrest' of granulocyte maturation at the myelocyte stage. Agar colony growth of marrow cells from each patient was subnormal but increased after removal of sheep erythrocyte rosette-forming cells (thymus-dependent [T] cells) from marrow cell suspensions before culture. Preincubation of marrow cells with cortisol also enhanced colony growth. Maximum enhancement with cortisol occurred at 1 mM (patient 1), 1 μM (patient 2), and 10 nM (patient 3). Cortisol failed to enhance colony growth after removal of T cells from marrow cell suspensions. Peripheral blood lymphocytes (PBL) and PBL-conditioned medium from all three patients inhibited colony growth of normal human marrow cells. Cortisol tretment of PBL or T depletion from PBL abrogated the inhibition seen both in coculture and with conditioned medium. Prednisone therapy resulted in the disappearance of suppressor T-cell function concomitant with hematologic improvement in patients 2 and 3, but suppressor T cells persisted in patient 1, who did not respond to prednisone. We conclude that cortisol-sensitive T lymphocytes inhibited granulopoiesis in vitro probably by elaboration of a soluble factor or factors. The results suggest that neutropenia in these patients resulted, at least in part, from T-cell suppression of granulopoiesis, the effectiveness of prednisone therapy was a result of its inhibition of suppressor T cells, and that responses to glucocorticoid therapy may be predicted in such patients with the agar culture technique and cortisol dose responses in vitro.

    Original languageEnglish (US)
    Pages (from-to)72-82
    Number of pages11
    JournalJournal of Clinical Investigation
    Volume64
    Issue number1
    StatePublished - 1979

    Fingerprint

    Neutropenia
    Hydrocortisone
    T-Lymphocytes
    Bone Marrow
    Prednisone
    Lymphocytes
    Conditioned Culture Medium
    Growth
    Agar
    Suspensions
    Felty Syndrome
    Polymyalgia Rheumatica
    Granulocyte Precursor Cells
    Culture Techniques
    Coculture Techniques
    Granulocytes
    Thymus Gland
    Glucocorticoids
    Sheep
    Therapeutics

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

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    abstract = "A man with polymyalgia rheumatica (patient 1) and two patients (2 and 3) with Felty's syndrome had neutropenia at the time of diagnosis. Bone marrow samples in each patient were cellular but showed an 'arrest' of granulocyte maturation at the myelocyte stage. Agar colony growth of marrow cells from each patient was subnormal but increased after removal of sheep erythrocyte rosette-forming cells (thymus-dependent [T] cells) from marrow cell suspensions before culture. Preincubation of marrow cells with cortisol also enhanced colony growth. Maximum enhancement with cortisol occurred at 1 mM (patient 1), 1 μM (patient 2), and 10 nM (patient 3). Cortisol failed to enhance colony growth after removal of T cells from marrow cell suspensions. Peripheral blood lymphocytes (PBL) and PBL-conditioned medium from all three patients inhibited colony growth of normal human marrow cells. Cortisol tretment of PBL or T depletion from PBL abrogated the inhibition seen both in coculture and with conditioned medium. Prednisone therapy resulted in the disappearance of suppressor T-cell function concomitant with hematologic improvement in patients 2 and 3, but suppressor T cells persisted in patient 1, who did not respond to prednisone. We conclude that cortisol-sensitive T lymphocytes inhibited granulopoiesis in vitro probably by elaboration of a soluble factor or factors. The results suggest that neutropenia in these patients resulted, at least in part, from T-cell suppression of granulopoiesis, the effectiveness of prednisone therapy was a result of its inhibition of suppressor T cells, and that responses to glucocorticoid therapy may be predicted in such patients with the agar culture technique and cortisol dose responses in vitro.",
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    AU - Gabourel, J. D.

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    N2 - A man with polymyalgia rheumatica (patient 1) and two patients (2 and 3) with Felty's syndrome had neutropenia at the time of diagnosis. Bone marrow samples in each patient were cellular but showed an 'arrest' of granulocyte maturation at the myelocyte stage. Agar colony growth of marrow cells from each patient was subnormal but increased after removal of sheep erythrocyte rosette-forming cells (thymus-dependent [T] cells) from marrow cell suspensions before culture. Preincubation of marrow cells with cortisol also enhanced colony growth. Maximum enhancement with cortisol occurred at 1 mM (patient 1), 1 μM (patient 2), and 10 nM (patient 3). Cortisol failed to enhance colony growth after removal of T cells from marrow cell suspensions. Peripheral blood lymphocytes (PBL) and PBL-conditioned medium from all three patients inhibited colony growth of normal human marrow cells. Cortisol tretment of PBL or T depletion from PBL abrogated the inhibition seen both in coculture and with conditioned medium. Prednisone therapy resulted in the disappearance of suppressor T-cell function concomitant with hematologic improvement in patients 2 and 3, but suppressor T cells persisted in patient 1, who did not respond to prednisone. We conclude that cortisol-sensitive T lymphocytes inhibited granulopoiesis in vitro probably by elaboration of a soluble factor or factors. The results suggest that neutropenia in these patients resulted, at least in part, from T-cell suppression of granulopoiesis, the effectiveness of prednisone therapy was a result of its inhibition of suppressor T cells, and that responses to glucocorticoid therapy may be predicted in such patients with the agar culture technique and cortisol dose responses in vitro.

    AB - A man with polymyalgia rheumatica (patient 1) and two patients (2 and 3) with Felty's syndrome had neutropenia at the time of diagnosis. Bone marrow samples in each patient were cellular but showed an 'arrest' of granulocyte maturation at the myelocyte stage. Agar colony growth of marrow cells from each patient was subnormal but increased after removal of sheep erythrocyte rosette-forming cells (thymus-dependent [T] cells) from marrow cell suspensions before culture. Preincubation of marrow cells with cortisol also enhanced colony growth. Maximum enhancement with cortisol occurred at 1 mM (patient 1), 1 μM (patient 2), and 10 nM (patient 3). Cortisol failed to enhance colony growth after removal of T cells from marrow cell suspensions. Peripheral blood lymphocytes (PBL) and PBL-conditioned medium from all three patients inhibited colony growth of normal human marrow cells. Cortisol tretment of PBL or T depletion from PBL abrogated the inhibition seen both in coculture and with conditioned medium. Prednisone therapy resulted in the disappearance of suppressor T-cell function concomitant with hematologic improvement in patients 2 and 3, but suppressor T cells persisted in patient 1, who did not respond to prednisone. We conclude that cortisol-sensitive T lymphocytes inhibited granulopoiesis in vitro probably by elaboration of a soluble factor or factors. The results suggest that neutropenia in these patients resulted, at least in part, from T-cell suppression of granulopoiesis, the effectiveness of prednisone therapy was a result of its inhibition of suppressor T cells, and that responses to glucocorticoid therapy may be predicted in such patients with the agar culture technique and cortisol dose responses in vitro.

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