Neutralizing antibody directed against the HIV-1 envelope glycoprotein can completely block HIV-1/SIV chimetic virus infections of macaque monkeys

Riri Shibata, Tatsuhiko Igarashi, Nancy Haigwood, Alicia Buckler-White, Robert Ogert, William Ross, Ronald Willey, Michael W. Cho, Malcolm A. Martin

    Research output: Contribution to journalArticle

    490 Scopus citations

    Abstract

    Virus-specific antibodies protect individuals against a wide variety of viral infections. To assess whether human immunodeficiency virus type 1 (HIV- 1) envelope-specific antibodies confer resistance against primate lentivirus infections, we purified immunoglobulin (IgG) from chimpanzees infected with several different HIV-1 isolates, and used this for passive immunization of pig-tailed macaques. These monkeys were subsequently challenged intravenously with a chimeric simian-human immunodeficiency virus (SHIV) bearing an envelope glycoprotein derived form HIV-1(DH12), a dual-tropic primary virus isolate. Here we show that anti-SHIV neutralizing activity, determined in vitro using an assay measuring loss of infectivity, is the absolute requirement for antibody-mediated protection in vivo. Using an assay that measures 100% neutralization, the titer in plasma for complete protection of the SHIV-challenged macaques was in the range of 1:5-1:8. The HIV-1-specific neutralizing antibodies studied are able to bind to native gp120 present on infectious virus particles. Administration of non-neutralizing anti-HIV IgG neither inhibited nor enhanced a subsequent SHIV infection.

    Original languageEnglish (US)
    Pages (from-to)204-210
    Number of pages7
    JournalNature medicine
    Volume5
    Issue number2
    DOIs
    StatePublished - Feb 1 1999

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

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