Neutralization of divergent HIV-1 isolates by conformation-dependent human antibodies to Gp120

Kathelyn S. Steimer, Carl J. Scandella, Paul V. Skiles, Nancy Haigwood

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

The spectrum of human immunodeficiency virus type 1 (HIV-1) isolates neutralized by antibodies from HIV-1-infected humans is broader than the spectrum of isolates neutralized by sera from animals immunized with purified gp120 subunits. This broader neutralization was due, in part, to the presence of antibodies to conserved gp120 conformational epitopes. Purified conformation-dependent gp120-specific human antibodies neutralized a wider range of virus isolates than human antibodies directed to linear determinants in gp120 and were also responsible for the majority of the gp120-specific CD4-blocking activity of HIV-1-infected human sera. A gp120 subunit vaccine that effectively presents these conformation-dependent neutralization epitopes should protect against a broader range of HIV-1 variants than a vaccine that presents exclusively linear determinants.

Original languageEnglish (US)
Pages (from-to)105-108
Number of pages4
JournalScience
Volume254
Issue number5028
StatePublished - Oct 4 1991
Externally publishedYes

Fingerprint

HIV-1
Antibodies
Epitopes
Subunit Vaccines
Serum
Vaccines
Viruses

ASJC Scopus subject areas

  • General

Cite this

Steimer, K. S., Scandella, C. J., Skiles, P. V., & Haigwood, N. (1991). Neutralization of divergent HIV-1 isolates by conformation-dependent human antibodies to Gp120. Science, 254(5028), 105-108.

Neutralization of divergent HIV-1 isolates by conformation-dependent human antibodies to Gp120. / Steimer, Kathelyn S.; Scandella, Carl J.; Skiles, Paul V.; Haigwood, Nancy.

In: Science, Vol. 254, No. 5028, 04.10.1991, p. 105-108.

Research output: Contribution to journalArticle

Steimer, KS, Scandella, CJ, Skiles, PV & Haigwood, N 1991, 'Neutralization of divergent HIV-1 isolates by conformation-dependent human antibodies to Gp120', Science, vol. 254, no. 5028, pp. 105-108.
Steimer, Kathelyn S. ; Scandella, Carl J. ; Skiles, Paul V. ; Haigwood, Nancy. / Neutralization of divergent HIV-1 isolates by conformation-dependent human antibodies to Gp120. In: Science. 1991 ; Vol. 254, No. 5028. pp. 105-108.
@article{176be4d349444af0900dea4dda6640fb,
title = "Neutralization of divergent HIV-1 isolates by conformation-dependent human antibodies to Gp120",
abstract = "The spectrum of human immunodeficiency virus type 1 (HIV-1) isolates neutralized by antibodies from HIV-1-infected humans is broader than the spectrum of isolates neutralized by sera from animals immunized with purified gp120 subunits. This broader neutralization was due, in part, to the presence of antibodies to conserved gp120 conformational epitopes. Purified conformation-dependent gp120-specific human antibodies neutralized a wider range of virus isolates than human antibodies directed to linear determinants in gp120 and were also responsible for the majority of the gp120-specific CD4-blocking activity of HIV-1-infected human sera. A gp120 subunit vaccine that effectively presents these conformation-dependent neutralization epitopes should protect against a broader range of HIV-1 variants than a vaccine that presents exclusively linear determinants.",
author = "Steimer, {Kathelyn S.} and Scandella, {Carl J.} and Skiles, {Paul V.} and Nancy Haigwood",
year = "1991",
month = "10",
day = "4",
language = "English (US)",
volume = "254",
pages = "105--108",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5028",

}

TY - JOUR

T1 - Neutralization of divergent HIV-1 isolates by conformation-dependent human antibodies to Gp120

AU - Steimer, Kathelyn S.

AU - Scandella, Carl J.

AU - Skiles, Paul V.

AU - Haigwood, Nancy

PY - 1991/10/4

Y1 - 1991/10/4

N2 - The spectrum of human immunodeficiency virus type 1 (HIV-1) isolates neutralized by antibodies from HIV-1-infected humans is broader than the spectrum of isolates neutralized by sera from animals immunized with purified gp120 subunits. This broader neutralization was due, in part, to the presence of antibodies to conserved gp120 conformational epitopes. Purified conformation-dependent gp120-specific human antibodies neutralized a wider range of virus isolates than human antibodies directed to linear determinants in gp120 and were also responsible for the majority of the gp120-specific CD4-blocking activity of HIV-1-infected human sera. A gp120 subunit vaccine that effectively presents these conformation-dependent neutralization epitopes should protect against a broader range of HIV-1 variants than a vaccine that presents exclusively linear determinants.

AB - The spectrum of human immunodeficiency virus type 1 (HIV-1) isolates neutralized by antibodies from HIV-1-infected humans is broader than the spectrum of isolates neutralized by sera from animals immunized with purified gp120 subunits. This broader neutralization was due, in part, to the presence of antibodies to conserved gp120 conformational epitopes. Purified conformation-dependent gp120-specific human antibodies neutralized a wider range of virus isolates than human antibodies directed to linear determinants in gp120 and were also responsible for the majority of the gp120-specific CD4-blocking activity of HIV-1-infected human sera. A gp120 subunit vaccine that effectively presents these conformation-dependent neutralization epitopes should protect against a broader range of HIV-1 variants than a vaccine that presents exclusively linear determinants.

UR - http://www.scopus.com/inward/record.url?scp=0026044947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026044947&partnerID=8YFLogxK

M3 - Article

C2 - 1718036

AN - SCOPUS:0026044947

VL - 254

SP - 105

EP - 108

JO - Science

JF - Science

SN - 0036-8075

IS - 5028

ER -