Neurotrophin-4/5 and neurotrophin-3 are present within the human ovarian follicle but appear to have different paracrine/autocrine functions

David Seifer, Bo Feng, Robert M. Shelden, Shiling Chen, Cheryl F. Dreyfus

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Neurotrophins are a family of soluble polypeptide growth factors widely recognized for their role in the mammalian nervous system. We first reported the unique presence of one neurotrophin, brain-derived neurotrophic factor (BDNF), in the follicular fluid of the human ovarian follicle. The BDNF receptor, Trk B, was identified in mouse oocytes, and BDNF accelerated first polar body extrusion in vitro. In the present study, we examined human follicular fluid and mouse immature oocytes to determine whether another Trk B ligand, neurotrophin-4/5 (NT-4/5), is present within the ovarian follicle and if so, whether it demonstrates activity similar to that of BDNF. We also examined whether a non-Trk B neurotrophin ligand, neurotrophin-3 (NT-3), is present within the follicle and has a possible role in oocyte maturation. NT-4/5 and NT-3 were noted to be present in all human follicular fluid samples aspirated from follicles of women undergoing in vitro fertilization. NT-4/5, but not NT-3, significantly promoted mouse oocyte polar body extrusion. Trk C receptors were not noted to be present in mouse oocytes. This study demonstrates for the first time that NT-4/5 and NT-3 are present in the follicular fluid of the human ovary. These data suggest that NT-4/5, like BDNF, promotes oocyte nuclear maturation. In contrast, NT-3 does not promote oocyte maturation but may contribute to follicle-oocyte maturation by mechanisms not yet identified.

Original languageEnglish (US)
Pages (from-to)4569-4571
Number of pages3
JournalJournal of Clinical Endocrinology and Metabolism
Volume87
Issue number10
DOIs
StatePublished - Oct 1 2002
Externally publishedYes

Fingerprint

Neurotrophin 3
Ovarian Follicle
Oocytes
Brain-Derived Neurotrophic Factor
Nerve Growth Factors
Follicular Fluid
Fluids
Extrusion
Polar Bodies
trkB Receptor
Ligands
Neurology
Intercellular Signaling Peptides and Proteins
neurotrophin 4
neurotrophin 5
Fertilization in Vitro
Peptides
Nervous System
Ovary

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Neurotrophin-4/5 and neurotrophin-3 are present within the human ovarian follicle but appear to have different paracrine/autocrine functions. / Seifer, David; Feng, Bo; Shelden, Robert M.; Chen, Shiling; Dreyfus, Cheryl F.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 87, No. 10, 01.10.2002, p. 4569-4571.

Research output: Contribution to journalArticle

Seifer, David ; Feng, Bo ; Shelden, Robert M. ; Chen, Shiling ; Dreyfus, Cheryl F. / Neurotrophin-4/5 and neurotrophin-3 are present within the human ovarian follicle but appear to have different paracrine/autocrine functions. In: Journal of Clinical Endocrinology and Metabolism. 2002 ; Vol. 87, No. 10. pp. 4569-4571.
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