Neuronal microtubule-associated protein 2D is a dual A-kinase anchoring protein expressed in rat ovarian granulosa cells

Lisa M. Salvador, Maxfield P. Flynn, Jesús Avila, Scott Reierstad, Evelyn T. Maizels, Hena Alam, Youngkyu Park, John D. Scott, Daniel Carr, Mary Hunzicker-Dunn

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Abstract

A-kinase anchoring proteins (AKAPs) function to target protein kinase A (PKA) to specific locations within the cell. AKAPs are functionally identified by their ability to bind the type II regulatory subunits (RII) of PKA in an in vitro overlay assay. We previously showed that follicle-stimulating hormone (FSH) induces the expression of an 80-kDa AKAP (AKAP 80) in ovarian granulosa cells as they mature from a preantral to a preovulatory phenotype. In this report, we identify AKAP 80 as microtubule-associated protein 2D (MAP2D), a low molecular weight splice variant of the neuronal MAP2 protein. MAP2D is induced in granulosa cells by dexamethasone and by FSH in a time-dependent manner that mimics that of AKAP 80, and immunoprecipitation of MAP2D depletes extracts of AKAP 80. MAP2D is the only MAP2 protein present in ovaries and is localized to granulosa cells of preovulatory follicles and to luteal cells. MAP2D is concentrated at the Golgi apparatus along with RI and RII and, based on coimmunoprecipitation results, appears to bind both RI and RII in granulosa cells. Reduced expression of MAP2D resulting from treatment of granulosa cells with antisense oligonucleotides to MAP2 inhibited the phosphorylation of cAMP-response element-binding protein. These results suggest that this classic neuronal RII AKAP is a dual RI/RII AKAP that performs unique functions in ovarian granulosa cells that contribute to the preovulatory phenotype.

Original languageEnglish (US)
Pages (from-to)27621-27632
Number of pages12
JournalJournal of Biological Chemistry
Volume279
Issue number26
DOIs
StatePublished - Jun 25 2004

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Microtubule-Associated Proteins
Granulosa Cells
Protein Kinases
Rats
Phosphotransferases
Proteins
Follicle Stimulating Hormone
Cyclic AMP-Dependent Protein Kinases
Phenotype
Luteal Cells
Cyclic AMP Response Element-Binding Protein
Antisense Oligonucleotides
Staphylococcal Protein A
Golgi Apparatus
Immunoprecipitation
Phosphorylation
Dexamethasone
Ovary
Molecular Weight
Assays

ASJC Scopus subject areas

  • Biochemistry

Cite this

Salvador, L. M., Flynn, M. P., Avila, J., Reierstad, S., Maizels, E. T., Alam, H., ... Hunzicker-Dunn, M. (2004). Neuronal microtubule-associated protein 2D is a dual A-kinase anchoring protein expressed in rat ovarian granulosa cells. Journal of Biological Chemistry, 279(26), 27621-27632. https://doi.org/10.1074/jbc.M402980200

Neuronal microtubule-associated protein 2D is a dual A-kinase anchoring protein expressed in rat ovarian granulosa cells. / Salvador, Lisa M.; Flynn, Maxfield P.; Avila, Jesús; Reierstad, Scott; Maizels, Evelyn T.; Alam, Hena; Park, Youngkyu; Scott, John D.; Carr, Daniel; Hunzicker-Dunn, Mary.

In: Journal of Biological Chemistry, Vol. 279, No. 26, 25.06.2004, p. 27621-27632.

Research output: Contribution to journalArticle

Salvador, LM, Flynn, MP, Avila, J, Reierstad, S, Maizels, ET, Alam, H, Park, Y, Scott, JD, Carr, D & Hunzicker-Dunn, M 2004, 'Neuronal microtubule-associated protein 2D is a dual A-kinase anchoring protein expressed in rat ovarian granulosa cells', Journal of Biological Chemistry, vol. 279, no. 26, pp. 27621-27632. https://doi.org/10.1074/jbc.M402980200
Salvador, Lisa M. ; Flynn, Maxfield P. ; Avila, Jesús ; Reierstad, Scott ; Maizels, Evelyn T. ; Alam, Hena ; Park, Youngkyu ; Scott, John D. ; Carr, Daniel ; Hunzicker-Dunn, Mary. / Neuronal microtubule-associated protein 2D is a dual A-kinase anchoring protein expressed in rat ovarian granulosa cells. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 26. pp. 27621-27632.
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