TY - JOUR
T1 - Neuron-Glia interactions through the heartless fgf receptor signaling pathway mediate morphogenesis of drosophila astrocytes
AU - Stork, Tobias
AU - Sheehan, Amy
AU - Tasdemir-Yilmaz, Ozge E.
AU - Freeman, Marc R.
N1 - Funding Information:
We are grateful to Christian Klämbt, Angelike Stathopoulos, Arno Müller, Ingolf Reim, Matthias Landgraf, Hermann Aberle, Marion Silies, Melissa Rolls, Tzumin Lee, Motojiro Yoshihara, Joachim Schulz, Gerd Vorbrüggen, Roger Jacobs as well as the Vienna Drosophila RNAi Center, the TRiP at Harvard Medical School (NIH/NIGMS R01-GM084947), and the Bloomington Stock Center for generously providing fly stocks. The monoclonal antibodies mAb 1D4, mAb 8D12, mab MR1A, and mAb nc82 developed by C. Goodman, C.Q. Doe, and E. Buchner, respectively, were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biological Sciences. Plasmids containing QF, codon-optimized Gal4, and iGluSnFR were obtained from Addgene. Roger Tsien generously provided a plasmid containing mCherry sequence and Tzumin Lee a plasmid containing LexA::GAD. We thank Looren Looger for generously sharing a GCaMP5a construct prior to publication. We further thank Michael Brodsky and the University of Massachusetts Medical School Mutagenesis Core for assistance with TALEN-mediated mutagenesis, the University of Massachusetts Medical School Electron Microscopy core facility for assistance with TEM experiments (supported by award number S10RR027897 from the National Center For Research Resources), Harleen Saini for help with laser injuries of astrocytes, and Johnna Doherty for generating a ths cDNA. T.S. was supported by a postdoctoral fellowship by the Deutsche Forschungsgemeinschaft (DFG). This work was supported by NINDS grant R01NS053538 (to M.R.F.). M.R.F. is an Investigator with the Howard Hughes Medical Institute.
PY - 2014/7/16
Y1 - 2014/7/16
N2 - Astrocytes are critically important for neuronal circuit assembly and function. Mammalian protoplasmic astrocytes develop a dense ramified meshwork of cellular processes to form intimate contacts with neuronal cell bodies, neurites, and synapses. This close neuron-glia morphological relationship is essential for astrocyte function, but it remains unclear how astrocytes establish their intricate morphology, organize spatial domains, and associate with neurons and synapses invivo. Here we characterize a Drosophila glial subtype that shows striking morphological and functional similarities to mammalian astrocytes. We demonstrate that the Fibroblast growth factor (FGF) receptor Heartless autonomously controls astrocyte membrane growth, and the FGFs Pyramus and Thisbe direct astrocyte processes to ramify specifically in CNS synaptic regions. We further show that the shape and size of individual astrocytes are dynamically sculpted through inhibitory or competitive astrocyte-astrocyte interactions and Heartless FGF signaling. Our data identify FGF signaling through Heartless as a key regulator of astrocyte morphological elaboration invivo.
AB - Astrocytes are critically important for neuronal circuit assembly and function. Mammalian protoplasmic astrocytes develop a dense ramified meshwork of cellular processes to form intimate contacts with neuronal cell bodies, neurites, and synapses. This close neuron-glia morphological relationship is essential for astrocyte function, but it remains unclear how astrocytes establish their intricate morphology, organize spatial domains, and associate with neurons and synapses invivo. Here we characterize a Drosophila glial subtype that shows striking morphological and functional similarities to mammalian astrocytes. We demonstrate that the Fibroblast growth factor (FGF) receptor Heartless autonomously controls astrocyte membrane growth, and the FGFs Pyramus and Thisbe direct astrocyte processes to ramify specifically in CNS synaptic regions. We further show that the shape and size of individual astrocytes are dynamically sculpted through inhibitory or competitive astrocyte-astrocyte interactions and Heartless FGF signaling. Our data identify FGF signaling through Heartless as a key regulator of astrocyte morphological elaboration invivo.
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U2 - 10.1016/j.neuron.2014.06.026
DO - 10.1016/j.neuron.2014.06.026
M3 - Article
C2 - 25033182
AN - SCOPUS:84904354254
SN - 0896-6273
VL - 83
SP - 388
EP - 403
JO - Neuron
JF - Neuron
IS - 2
ER -