Neurodevelopmental and cognitive behavior of glutaryl-CoA dehydrogenase deficient knockout mice

Estela Natacha Brandt Busanello, Letícia Pettenuzzo, Paulo Henrique Botton, Pablo Pandolfo, Diogo Onofre Gomes De Souza, Michael Woontner, Stephen Goodman, David Koeller, Moacir Wajner

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5 Scopus citations

Abstract

Aims: The establishment of a genetic knockout murine model of glutaric acidemia type I (GAI) with complete loss of glutaryl-CoA dehydrogenase (GCDH) activity has been used to investigate the pathological mechanisms underlying neurological symptoms in this disorder. However, very little has been reported on the neurobehavior of GCDH deficient mice (Gcdh-/-). Main methods: In the present study we evaluated physical (body and weight gain) and neuromotor development (appearance of coat, upper incisor eruption, eye-opening day, motor coordination, muscular strength and climbing), as well as cognitive behavior (inhibitory avoidance) in Gcdh-/-, as compared to wild type (WT) mice. Key findings: We found that Gcdh-/- mice did not differ in body and weight gain, appearance of coat, upper incisor eruption, motor coordination and muscular strength, but had a significant delayed eye opening, implying a mild impairment of neurodevelopment in these animals. Furthermore, the climbing behavior was significantly higher in Gcdh-/- as compared to WT mice, suggesting an altered dopaminergic function. Finally, Gcdh-/- mice presented a deficit of short- and long-term memories in the inhibitory avoidance task. Significance: Although it is difficult to extrapolate the present findings to the human condition, our present data are particularly interesting in view of the psychomotor/mental delay that occurs in a significant number of GAI patients with no previous history of acute encephalopathy with striatum destruction. Strict and early treatment possibly associated with novel therapies seems therefore important to prevent learning/memory disabilities in GAI patients.

Original languageEnglish (US)
Pages (from-to)137-142
Number of pages6
JournalLife Sciences
Volume92
Issue number2
DOIs
StatePublished - Feb 7 2013

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Keywords

  • Climbing behavior
  • Cognitive behavior
  • Glutaric acidemia type I
  • Knockout mice model
  • Mice behavior
  • Neurodevelopment

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Busanello, E. N. B., Pettenuzzo, L., Botton, P. H., Pandolfo, P., De Souza, D. O. G., Woontner, M., Goodman, S., Koeller, D., & Wajner, M. (2013). Neurodevelopmental and cognitive behavior of glutaryl-CoA dehydrogenase deficient knockout mice. Life Sciences, 92(2), 137-142. https://doi.org/10.1016/j.lfs.2012.11.013