Neural transplantation of human MSC and NT2 cells in the twitcher mouse model

J. Croitoru-Lamoury, K. R. Williams, F. M.J. Lamoury, L. A. Veas, B. Ajami, R. M. Taylor, B. J. Brew

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Background: Accumulating evidence has demonstrated that the NT2 embryonal carcinoma cell line and multipotential stem cells found in BM, mesenchymal stromal cells (MSC), have the ability to differentiate into a wide variety of cell types. This study was designed to explore the efficacy of these two human stem cell types as a graft source for the treatment of demyelinating disorders such as Krabbe's disease and multiple sclerosis (MS). Methods: We examined the engraftment and in vivo differentiation of adult MSC and NT2 cells after transplantation into two demyelinating environments, the neonatal and postnatal twitcher mouse brain. Results: Both types of xenografts led to anatomical integration, without tumor formation, and remained viable in the normal and twitcher mouse brain, showing differentiation into neurons, astrocytes and oligodendrocytes. Discussion: This study represents a platform for further stem cell transplantation studies in the twitcher model and potentially has important therapeutic implications.

Original languageEnglish (US)
Pages (from-to)445-458
Number of pages14
JournalCytotherapy
Volume8
Issue number5
DOIs
StatePublished - Nov 2006
Externally publishedYes

Keywords

  • Adult stem cells
  • Marrow stromal cells
  • Neural transplantation
  • Twitcher mouse

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Genetics(clinical)
  • Cell Biology
  • Cancer Research
  • Transplantation

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