Neural injury alters proliferation and integration of adult-generated neurons in the dentate gyrus

Julia V. Perederiy, Bryan W. Luikart, Eric K. Washburn, Eric Schnell, Gary Westbrook

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Neural plasticity following brain injury illustrates the potential for regeneration in the central nervous system. Lesioning of the perforant path, which innervates the outer two-thirds of the molecular layer of the dentate gyrus, was one of the first models to demonstrate structural plasticity of mature granule cells (Parnavelas et al., 1974; Caceres and Steward, 1983; Diekmann et al., 1996). The dentate gyrus also harbors a continuously proliferating population of neuronal precursors that can integrate into functional circuits and show enhanced short-term plasticity (Schmidt-Hieber et al., 2004; Abrous et al., 2005). To examine the response of adult-generated granule cells to unilateral complete transection of the perforant path in vivo, we tracked these cells using transgenic POMC-EGFP mice or by retroviral expression of GFP. Lesioning triggered a marked proliferation of newborn neurons. Subsequently, the dendrites of newborn neurons showed reduced complexity within the denervated zone, but dendritic spines still formed in the absence of glutamatergic nerve terminals. Electron micrographs confirmed the lack of intact presynaptic terminals apposing spines on mature cells and on newborn neurons. Newborn neurons, but not mature granule cells, had a higher density of dendritic spines in the inner molecular layer postlesion accompanied by an increase in miniature EPSC amplitudes and rise times. Our results indicate that injury causes an increase in newborn neurons and lamina-specific synaptic reorganization indicative of enhanced plasticity. The presence of de novo dendritic spines in the denervated zone suggests that the postlesion environment provides the necessary signals for spine formation.

Original languageEnglish (US)
Pages (from-to)4754-4767
Number of pages14
JournalJournal of Neuroscience
Volume33
Issue number11
DOIs
StatePublished - Mar 13 2013

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Dentate Gyrus
Dendritic Spines
Neurons
Wounds and Injuries
Perforant Pathway
Spine
Pro-Opiomelanocortin
Neuronal Plasticity
Presynaptic Terminals
Dendrites
Brain Injuries
Regeneration
Central Nervous System
Electrons
Population

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neural injury alters proliferation and integration of adult-generated neurons in the dentate gyrus. / Perederiy, Julia V.; Luikart, Bryan W.; Washburn, Eric K.; Schnell, Eric; Westbrook, Gary.

In: Journal of Neuroscience, Vol. 33, No. 11, 13.03.2013, p. 4754-4767.

Research output: Contribution to journalArticle

Perederiy, Julia V. ; Luikart, Bryan W. ; Washburn, Eric K. ; Schnell, Eric ; Westbrook, Gary. / Neural injury alters proliferation and integration of adult-generated neurons in the dentate gyrus. In: Journal of Neuroscience. 2013 ; Vol. 33, No. 11. pp. 4754-4767.
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