Network analysis of the focal adhesion to invadopodia transition identifies a PI3K-PKCα invasive signaling axis

Daisuke Hoshino, Jerome Jourquin, Shane Weller Emmons, Tyne Miller, Margalit Goldgof, Kaitlin Costello, Darren R. Tyson, Brandee Brown, Yiling Lu, Nagendra K. Prasad, Bing Zhang, Gordon Mills, Wendell G. Yarbrough, Vito Quaranta, Motoharu Seiki, Alissa M. Weaver

Research output: Contribution to journalArticle

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Abstract

In cancer, deregulated signaling can produce an invasive cellular phenotype. We modeled the invasive transition as a theoretical switch between two cytoskeletal structures: focal adhesions and extracellular matrix-degrading invadopodia. We constructed molecular interaction networks of each structure and identified upstream regulatory hubs through computational analyses. We compared these regulatory hubs to the status of signaling components from head and neck carcinomas, which led us to analyze phosphatidylinositol 3-kinase (PI3K) and protein kinase C α (PKCα). Consistent with previous studies, PI3K activity promoted both the formation and the activity of invadopodia. We found that PI3K induction of invadopodia was increased by overexpression of SH2 (Src homology 2 domain) domain-containing inositol 5′-phosphatase 2 (SHIP2), which converts the phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P 3] that is produced by PI3K activity to phosphatidylinositol 3,4-bisphosphate [PI(3,4)P2], which is believed to promote invadopodia formation. Knockdown of PKCα had divergent effects on invadopodia formation, depending on the status of PI3K. Loss of PKCα inhibited invadopodia formation in cells with wild-type PI3K pathway status. Conversely, in cells with constitutively active PI3K (through activating PI3K mutants or lacking the endogenous opposing enzyme PTEN), PKCα knockdown increased invadopodia formation. Mechanistic studies revealed a negative feedback loop from PKCα that dampened PI3K activity and invasive behavior in cells with genetic hyperactivation of the PI3K pathway. These studies demonstrated the potential of network modeling as a discovery tool and identified PI3K and PKCα as interacting regulators of invasive behavior.

Original languageEnglish (US)
Article numberra66
JournalScience Signaling
Volume5
Issue number241
DOIs
StatePublished - Sep 11 2012
Externally publishedYes

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Phosphatidylinositol 3-Kinase
Focal Adhesions
Electric network analysis
Protein Kinase C
Adhesion
Podosomes
PTEN Phosphohydrolase
src Homology Domains
Molecular interactions
Inositol
Phosphoric Monoester Hydrolases
Extracellular Matrix
Neck

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hoshino, D., Jourquin, J., Emmons, S. W., Miller, T., Goldgof, M., Costello, K., ... Weaver, A. M. (2012). Network analysis of the focal adhesion to invadopodia transition identifies a PI3K-PKCα invasive signaling axis. Science Signaling, 5(241), [ra66]. https://doi.org/10.1126/scisignal.2002964

Network analysis of the focal adhesion to invadopodia transition identifies a PI3K-PKCα invasive signaling axis. / Hoshino, Daisuke; Jourquin, Jerome; Emmons, Shane Weller; Miller, Tyne; Goldgof, Margalit; Costello, Kaitlin; Tyson, Darren R.; Brown, Brandee; Lu, Yiling; Prasad, Nagendra K.; Zhang, Bing; Mills, Gordon; Yarbrough, Wendell G.; Quaranta, Vito; Seiki, Motoharu; Weaver, Alissa M.

In: Science Signaling, Vol. 5, No. 241, ra66, 11.09.2012.

Research output: Contribution to journalArticle

Hoshino, D, Jourquin, J, Emmons, SW, Miller, T, Goldgof, M, Costello, K, Tyson, DR, Brown, B, Lu, Y, Prasad, NK, Zhang, B, Mills, G, Yarbrough, WG, Quaranta, V, Seiki, M & Weaver, AM 2012, 'Network analysis of the focal adhesion to invadopodia transition identifies a PI3K-PKCα invasive signaling axis', Science Signaling, vol. 5, no. 241, ra66. https://doi.org/10.1126/scisignal.2002964
Hoshino, Daisuke ; Jourquin, Jerome ; Emmons, Shane Weller ; Miller, Tyne ; Goldgof, Margalit ; Costello, Kaitlin ; Tyson, Darren R. ; Brown, Brandee ; Lu, Yiling ; Prasad, Nagendra K. ; Zhang, Bing ; Mills, Gordon ; Yarbrough, Wendell G. ; Quaranta, Vito ; Seiki, Motoharu ; Weaver, Alissa M. / Network analysis of the focal adhesion to invadopodia transition identifies a PI3K-PKCα invasive signaling axis. In: Science Signaling. 2012 ; Vol. 5, No. 241.
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