Nerve growth factor regulates synaptophysin expression in developing trigeminal ganglion neurons in vitro

L. Tarsa, A. Balkowiec

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The role of neuronal growth factors in synaptic maturation of sensory neurons, including trigeminal ganglion (TG) neurons, remains poorly understood. Here, we show that nerve growth factor (NGF) regulates the intracellular distribution of the synaptic vesicle protein synaptophysin (Syp) in newborn rat TG neurons in vitro. While reducing the number of Syp-positive cell bodies, NGF dramatically increases Syp immunoreactivity in both proximal and distal segments of the neurite. Intriguingly, the increase in Syp immunoreactivity occurs only in neuron-enriched cultures, in which the number of non-neuronal cells is significantly reduced. Together, our data indicate that NGF is a candidate molecule involved in early postnatal maturation of TG neurons, including control of presynaptic assembly, and thereby formation of synaptic connections.

Original languageEnglish (US)
Pages (from-to)47-52
Number of pages6
JournalNeuropeptides
Volume43
Issue number1
DOIs
StatePublished - Feb 1 2009

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Keywords

  • Development
  • In vitro
  • Nerve growth factor
  • Sensory neuron
  • Synaptophysin
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Endocrinology
  • Neurology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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