Nerve growth factor is required for early follicular development in the mammalian ovary

Gregory Dissen, Carmen Romero, Anne Newman Hirshfield, Sergio Ojeda

    Research output: Contribution to journalArticle

    162 Citations (Scopus)

    Abstract

    Nerve growth factor (NGF) epitomizes a family of proteins known as the neurotrophins (NTs), which are required for the survival and differentiation of neurons within both the central and peripheral nervous system. Synthesis of NGF in tissues innervated by the peripheral nervous system is consistent with its function as a target-derived trophic factor. However, the presence of low- and high-affinity NGF receptors in the gonads suggests another function for the NTs within the reproductive endocrine system. We now report that NGF is required for the growth of primordial ovarian follicles, a process known to occur independently of pituitary gonadotropins. Both the NT receptor p75NTR and the NGF tyrosine kinase receptor trkA were found to be expressed in the ovaries of infantile normal mice and mice carrying a null mutation of the NGF gene. The ovaries from homozygote NGF-null (-/-) mutant animals, analyzed after completion of ovarian histogenesis, exhibited a markedly reduced population of primary and secondary follicles in the presence of normal serum gonadotropin levels, and an increased number of oocytes that failed to be incorporated into a follicular structure. Assessment of mitogenic activity using two complementary proliferation markers revealed a conspicuous reduction in somatic cell proliferation in the ovaries of NGF-deficient mice. These results suggest that the delay in follicular growth observed in NGF-/- mice may be related to the loss of a proliferative signal provided by NGF to the nonneural endocrine component of the ovary.

    Original languageEnglish (US)
    Pages (from-to)2078-2086
    Number of pages9
    JournalEndocrinology
    Volume142
    Issue number5
    DOIs
    StatePublished - 2001

    Fingerprint

    Nerve Growth Factor
    Ovary
    Nerve Growth Factor Receptors
    Peripheral Nervous System
    Nerve Growth Factors
    Pituitary Gonadotropins
    Endocrine System
    Ovarian Follicle
    Homozygote
    Gonads
    Receptor Protein-Tyrosine Kinases
    Growth
    Gonadotropins
    Oocytes
    Central Nervous System
    Cell Proliferation
    Neurons
    Mutation
    Serum

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Nerve growth factor is required for early follicular development in the mammalian ovary. / Dissen, Gregory; Romero, Carmen; Hirshfield, Anne Newman; Ojeda, Sergio.

    In: Endocrinology, Vol. 142, No. 5, 2001, p. 2078-2086.

    Research output: Contribution to journalArticle

    Dissen, Gregory ; Romero, Carmen ; Hirshfield, Anne Newman ; Ojeda, Sergio. / Nerve growth factor is required for early follicular development in the mammalian ovary. In: Endocrinology. 2001 ; Vol. 142, No. 5. pp. 2078-2086.
    @article{9ca07fc482b34be9b5da986ac57e079c,
    title = "Nerve growth factor is required for early follicular development in the mammalian ovary",
    abstract = "Nerve growth factor (NGF) epitomizes a family of proteins known as the neurotrophins (NTs), which are required for the survival and differentiation of neurons within both the central and peripheral nervous system. Synthesis of NGF in tissues innervated by the peripheral nervous system is consistent with its function as a target-derived trophic factor. However, the presence of low- and high-affinity NGF receptors in the gonads suggests another function for the NTs within the reproductive endocrine system. We now report that NGF is required for the growth of primordial ovarian follicles, a process known to occur independently of pituitary gonadotropins. Both the NT receptor p75NTR and the NGF tyrosine kinase receptor trkA were found to be expressed in the ovaries of infantile normal mice and mice carrying a null mutation of the NGF gene. The ovaries from homozygote NGF-null (-/-) mutant animals, analyzed after completion of ovarian histogenesis, exhibited a markedly reduced population of primary and secondary follicles in the presence of normal serum gonadotropin levels, and an increased number of oocytes that failed to be incorporated into a follicular structure. Assessment of mitogenic activity using two complementary proliferation markers revealed a conspicuous reduction in somatic cell proliferation in the ovaries of NGF-deficient mice. These results suggest that the delay in follicular growth observed in NGF-/- mice may be related to the loss of a proliferative signal provided by NGF to the nonneural endocrine component of the ovary.",
    author = "Gregory Dissen and Carmen Romero and Hirshfield, {Anne Newman} and Sergio Ojeda",
    year = "2001",
    doi = "10.1210/en.142.5.2078",
    language = "English (US)",
    volume = "142",
    pages = "2078--2086",
    journal = "Endocrinology",
    issn = "0013-7227",
    publisher = "The Endocrine Society",
    number = "5",

    }

    TY - JOUR

    T1 - Nerve growth factor is required for early follicular development in the mammalian ovary

    AU - Dissen, Gregory

    AU - Romero, Carmen

    AU - Hirshfield, Anne Newman

    AU - Ojeda, Sergio

    PY - 2001

    Y1 - 2001

    N2 - Nerve growth factor (NGF) epitomizes a family of proteins known as the neurotrophins (NTs), which are required for the survival and differentiation of neurons within both the central and peripheral nervous system. Synthesis of NGF in tissues innervated by the peripheral nervous system is consistent with its function as a target-derived trophic factor. However, the presence of low- and high-affinity NGF receptors in the gonads suggests another function for the NTs within the reproductive endocrine system. We now report that NGF is required for the growth of primordial ovarian follicles, a process known to occur independently of pituitary gonadotropins. Both the NT receptor p75NTR and the NGF tyrosine kinase receptor trkA were found to be expressed in the ovaries of infantile normal mice and mice carrying a null mutation of the NGF gene. The ovaries from homozygote NGF-null (-/-) mutant animals, analyzed after completion of ovarian histogenesis, exhibited a markedly reduced population of primary and secondary follicles in the presence of normal serum gonadotropin levels, and an increased number of oocytes that failed to be incorporated into a follicular structure. Assessment of mitogenic activity using two complementary proliferation markers revealed a conspicuous reduction in somatic cell proliferation in the ovaries of NGF-deficient mice. These results suggest that the delay in follicular growth observed in NGF-/- mice may be related to the loss of a proliferative signal provided by NGF to the nonneural endocrine component of the ovary.

    AB - Nerve growth factor (NGF) epitomizes a family of proteins known as the neurotrophins (NTs), which are required for the survival and differentiation of neurons within both the central and peripheral nervous system. Synthesis of NGF in tissues innervated by the peripheral nervous system is consistent with its function as a target-derived trophic factor. However, the presence of low- and high-affinity NGF receptors in the gonads suggests another function for the NTs within the reproductive endocrine system. We now report that NGF is required for the growth of primordial ovarian follicles, a process known to occur independently of pituitary gonadotropins. Both the NT receptor p75NTR and the NGF tyrosine kinase receptor trkA were found to be expressed in the ovaries of infantile normal mice and mice carrying a null mutation of the NGF gene. The ovaries from homozygote NGF-null (-/-) mutant animals, analyzed after completion of ovarian histogenesis, exhibited a markedly reduced population of primary and secondary follicles in the presence of normal serum gonadotropin levels, and an increased number of oocytes that failed to be incorporated into a follicular structure. Assessment of mitogenic activity using two complementary proliferation markers revealed a conspicuous reduction in somatic cell proliferation in the ovaries of NGF-deficient mice. These results suggest that the delay in follicular growth observed in NGF-/- mice may be related to the loss of a proliferative signal provided by NGF to the nonneural endocrine component of the ovary.

    UR - http://www.scopus.com/inward/record.url?scp=0035043232&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0035043232&partnerID=8YFLogxK

    U2 - 10.1210/en.142.5.2078

    DO - 10.1210/en.142.5.2078

    M3 - Article

    C2 - 11316775

    AN - SCOPUS:0035043232

    VL - 142

    SP - 2078

    EP - 2086

    JO - Endocrinology

    JF - Endocrinology

    SN - 0013-7227

    IS - 5

    ER -