Nephrotoxicity of the constituents of the gentamicin complex

S. J. Kohlhepp, M. O. Loveless, P. W. Kohnen, D. C. Houghton, W. M. Bennett, D. N. Gilbert

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Abstract

Commercial gentamicin C is a mixture of gentamicin C1, C(1a), and C2. The nephrotoxicity of each of these constitutents was compared with that of the gentamicin complex. After seven days the mean creatinine level in serum was 0.8 mg/dl in rats given C2 and 0.5 mg/dl in rats given C1, C(1a), or the gentamicin complex (P < .001). Toxicity attributable to C1a) was not detected until day 14, and only minimal toxicity was noted in C1-treated rats after 21 days. Nephrotoxicity caused by the gentamicin complex was similar to that caused by C2. By a new high-pressure liquid chromatographic method, the renal concentration of C1, C(1a), and C2 was quantified in rats given the gentamicin complex. The results indicated an early, preferential renal accumulation of C2. Subsequently, the C2 content of 12 commercial lots of gentamicin C was measured. The C2 concentration ranged from 12.4 to 10.1 mg/ml. In short, experimental nephrotoxicity from gentamicin C is largely the result of the C2 constituent, and the concentration of this constituent in commercial preparations of gentamicin varies by as much as 7.7 mg/dl.

Original languageEnglish (US)
Pages (from-to)605-614
Number of pages10
JournalJournal of Infectious Diseases
Volume149
Issue number4
DOIs
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Kohlhepp, S. J., Loveless, M. O., Kohnen, P. W., Houghton, D. C., Bennett, W. M., & Gilbert, D. N. (1984). Nephrotoxicity of the constituents of the gentamicin complex. Journal of Infectious Diseases, 149(4), 605-614. https://doi.org/10.1093/infdis/149.4.605