Nephrotoxicity of the constituents of the gentamicin complex

S. J. Kohlhepp, M. O. Loveless, P. W. Kohnen, Donald Houghton, W. M. Bennett, D. N. Gilbert

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Commercial gentamicin C is a mixture of gentamicin C1, C(1a), and C2. The nephrotoxicity of each of these constitutents was compared with that of the gentamicin complex. After seven days the mean creatinine level in serum was 0.8 mg/dl in rats given C2 and 0.5 mg/dl in rats given C1, C(1a), or the gentamicin complex (P <.001). Toxicity attributable to C1a) was not detected until day 14, and only minimal toxicity was noted in C1-treated rats after 21 days. Nephrotoxicity caused by the gentamicin complex was similar to that caused by C2. By a new high-pressure liquid chromatographic method, the renal concentration of C1, C(1a), and C2 was quantified in rats given the gentamicin complex. The results indicated an early, preferential renal accumulation of C2. Subsequently, the C2 content of 12 commercial lots of gentamicin C was measured. The C2 concentration ranged from 12.4 to 10.1 mg/ml. In short, experimental nephrotoxicity from gentamicin C is largely the result of the C2 constituent, and the concentration of this constituent in commercial preparations of gentamicin varies by as much as 7.7 mg/dl.

Original languageEnglish (US)
Pages (from-to)605-614
Number of pages10
JournalJournal of Infectious Diseases
Volume149
Issue number4
StatePublished - 1984

Fingerprint

Gentamicins
Kidney
Creatinine
gentamicin C
Pressure
Serum

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Kohlhepp, S. J., Loveless, M. O., Kohnen, P. W., Houghton, D., Bennett, W. M., & Gilbert, D. N. (1984). Nephrotoxicity of the constituents of the gentamicin complex. Journal of Infectious Diseases, 149(4), 605-614.

Nephrotoxicity of the constituents of the gentamicin complex. / Kohlhepp, S. J.; Loveless, M. O.; Kohnen, P. W.; Houghton, Donald; Bennett, W. M.; Gilbert, D. N.

In: Journal of Infectious Diseases, Vol. 149, No. 4, 1984, p. 605-614.

Research output: Contribution to journalArticle

Kohlhepp, SJ, Loveless, MO, Kohnen, PW, Houghton, D, Bennett, WM & Gilbert, DN 1984, 'Nephrotoxicity of the constituents of the gentamicin complex', Journal of Infectious Diseases, vol. 149, no. 4, pp. 605-614.
Kohlhepp SJ, Loveless MO, Kohnen PW, Houghton D, Bennett WM, Gilbert DN. Nephrotoxicity of the constituents of the gentamicin complex. Journal of Infectious Diseases. 1984;149(4):605-614.
Kohlhepp, S. J. ; Loveless, M. O. ; Kohnen, P. W. ; Houghton, Donald ; Bennett, W. M. ; Gilbert, D. N. / Nephrotoxicity of the constituents of the gentamicin complex. In: Journal of Infectious Diseases. 1984 ; Vol. 149, No. 4. pp. 605-614.
@article{2b4151afb7f24d43818d73854bba7e21,
title = "Nephrotoxicity of the constituents of the gentamicin complex",
abstract = "Commercial gentamicin C is a mixture of gentamicin C1, C(1a), and C2. The nephrotoxicity of each of these constitutents was compared with that of the gentamicin complex. After seven days the mean creatinine level in serum was 0.8 mg/dl in rats given C2 and 0.5 mg/dl in rats given C1, C(1a), or the gentamicin complex (P <.001). Toxicity attributable to C1a) was not detected until day 14, and only minimal toxicity was noted in C1-treated rats after 21 days. Nephrotoxicity caused by the gentamicin complex was similar to that caused by C2. By a new high-pressure liquid chromatographic method, the renal concentration of C1, C(1a), and C2 was quantified in rats given the gentamicin complex. The results indicated an early, preferential renal accumulation of C2. Subsequently, the C2 content of 12 commercial lots of gentamicin C was measured. The C2 concentration ranged from 12.4 to 10.1 mg/ml. In short, experimental nephrotoxicity from gentamicin C is largely the result of the C2 constituent, and the concentration of this constituent in commercial preparations of gentamicin varies by as much as 7.7 mg/dl.",
author = "Kohlhepp, {S. J.} and Loveless, {M. O.} and Kohnen, {P. W.} and Donald Houghton and Bennett, {W. M.} and Gilbert, {D. N.}",
year = "1984",
language = "English (US)",
volume = "149",
pages = "605--614",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Nephrotoxicity of the constituents of the gentamicin complex

AU - Kohlhepp, S. J.

AU - Loveless, M. O.

AU - Kohnen, P. W.

AU - Houghton, Donald

AU - Bennett, W. M.

AU - Gilbert, D. N.

PY - 1984

Y1 - 1984

N2 - Commercial gentamicin C is a mixture of gentamicin C1, C(1a), and C2. The nephrotoxicity of each of these constitutents was compared with that of the gentamicin complex. After seven days the mean creatinine level in serum was 0.8 mg/dl in rats given C2 and 0.5 mg/dl in rats given C1, C(1a), or the gentamicin complex (P <.001). Toxicity attributable to C1a) was not detected until day 14, and only minimal toxicity was noted in C1-treated rats after 21 days. Nephrotoxicity caused by the gentamicin complex was similar to that caused by C2. By a new high-pressure liquid chromatographic method, the renal concentration of C1, C(1a), and C2 was quantified in rats given the gentamicin complex. The results indicated an early, preferential renal accumulation of C2. Subsequently, the C2 content of 12 commercial lots of gentamicin C was measured. The C2 concentration ranged from 12.4 to 10.1 mg/ml. In short, experimental nephrotoxicity from gentamicin C is largely the result of the C2 constituent, and the concentration of this constituent in commercial preparations of gentamicin varies by as much as 7.7 mg/dl.

AB - Commercial gentamicin C is a mixture of gentamicin C1, C(1a), and C2. The nephrotoxicity of each of these constitutents was compared with that of the gentamicin complex. After seven days the mean creatinine level in serum was 0.8 mg/dl in rats given C2 and 0.5 mg/dl in rats given C1, C(1a), or the gentamicin complex (P <.001). Toxicity attributable to C1a) was not detected until day 14, and only minimal toxicity was noted in C1-treated rats after 21 days. Nephrotoxicity caused by the gentamicin complex was similar to that caused by C2. By a new high-pressure liquid chromatographic method, the renal concentration of C1, C(1a), and C2 was quantified in rats given the gentamicin complex. The results indicated an early, preferential renal accumulation of C2. Subsequently, the C2 content of 12 commercial lots of gentamicin C was measured. The C2 concentration ranged from 12.4 to 10.1 mg/ml. In short, experimental nephrotoxicity from gentamicin C is largely the result of the C2 constituent, and the concentration of this constituent in commercial preparations of gentamicin varies by as much as 7.7 mg/dl.

UR - http://www.scopus.com/inward/record.url?scp=0021364924&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021364924&partnerID=8YFLogxK

M3 - Article

C2 - 6725992

AN - SCOPUS:0021364924

VL - 149

SP - 605

EP - 614

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 4

ER -