Natriuretic peptides and nitric oxide stimulate cGMP synthesis in different cellular compartments

Leslie A. Piggott, Kathryn A. Hassell, Zuzana Berkova, Andrew P. Morris, Gary (Michael) Silberbach, Thomas C. Rich

Research output: Contribution to journalArticle

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Abstract

Cyclic nucleotide-gated (CNG) channels are a family of ion channels activated by the binding of cyclic nucleotides. Endogenous channels have been used to measure cyclic nucleotide signals in photoreceptor outer segments and olfactory cilia for decades. Here we have investigated the subcellular localization of cGMP signals by monitoring CNG channel activity in response to agonists that activate either particulate or soluble guanylyl cyclase. CNG channels were heterologously expressed in either human embryonic kidney (HEK)-293 cells that stably overexpress a particulate guanylyl cyclase (HEK-NPRA cells), or cultured vascular smooth muscle cells (VSMCs). Atrial natriuretic peptide (ANP) was used to activate the particulate guanylyl cyclase and the nitric oxide donor S-nitroso-n-acetylpenicillamine (SNAP) was used to activate the soluble guanylyl cyclase. CNG channel activity was monitored by measuring Ca2+ or Mn2+ influx through the channels using the fluorescent dye, fura-2. We found that in HEK-NPRA cells, ANP-induced increases in cGMP levels activated CNG channels in a dose-dependent manner (0.05-10 nM), whereas SNAP (0.01-100 μM) induced increases in cGMP levels triggered little or no activation of CNG channels (P <0.01). After pretreatment with 100 μM 3-isobutyl-1-methylxanthine (IBMX), a nonspecific phosphodiesterase inhibitor, ANP-induced Mn2+ influx through CNG channels was significantly enhanced, while SNAP-induced Mn2+ influx remained small. In contrast, we found that in the presence of IBMX, both 1 nM ANP and 100 μM SNAP triggered similar increases in total cGMP levels. We next sought to determine if cGMP signals are compartmentalized in VSMCs, which endogenously express particulate and soluble guanylyl cyclase. We found that 10 nM ANP induced activation of CNG channels more readily than 100 μM SNAP; whereas 100 μM SNAP triggered higher levels of total cellular cGMP accumulation. These results suggest that cGMP signals are spatially segregated within cells, and that the functional compartmentalization of cGMP signals may underlie the unique actions of ANP and nitric oxide.

Original languageEnglish (US)
Pages (from-to)3-14
Number of pages12
JournalJournal of General Physiology
Volume128
Issue number1
DOIs
StatePublished - 2006

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Cyclic Nucleotide-Gated Cation Channels
Natriuretic Peptides
Nitric Oxide
Atrial Natriuretic Factor
Guanylate Cyclase
Cyclic Nucleotides
Kidney
Vascular Smooth Muscle
Smooth Muscle Myocytes
1-Methyl-3-isobutylxanthine
Phosphodiesterase Inhibitors
Nitric Oxide Donors
Cilia
Fura-2
Ion Channels
Fluorescent Dyes
Cultured Cells

ASJC Scopus subject areas

  • Physiology

Cite this

Natriuretic peptides and nitric oxide stimulate cGMP synthesis in different cellular compartments. / Piggott, Leslie A.; Hassell, Kathryn A.; Berkova, Zuzana; Morris, Andrew P.; Silberbach, Gary (Michael); Rich, Thomas C.

In: Journal of General Physiology, Vol. 128, No. 1, 2006, p. 3-14.

Research output: Contribution to journalArticle

Piggott, Leslie A. ; Hassell, Kathryn A. ; Berkova, Zuzana ; Morris, Andrew P. ; Silberbach, Gary (Michael) ; Rich, Thomas C. / Natriuretic peptides and nitric oxide stimulate cGMP synthesis in different cellular compartments. In: Journal of General Physiology. 2006 ; Vol. 128, No. 1. pp. 3-14.
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AU - Rich, Thomas C.

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