Nascent proteomes in peripheral blood mononuclear cells as a novel source for biomarker discovery in human stroke

Fang Bian, Roger P. Simon, Yun Li, Larry David, Jolita Wainwright, Casey L. Hall, Michael Frankel, An Zhou

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

BACKGROUND AND PURPOSE - : The proteome of newly synthesized proteins (nascent proteome) in peripheral blood mononuclear cells (PBMCs) can be a novel source of stroke biomarkers. Changes in the PBMC nascent proteome after stroke reflect the dynamic response-in-action not detectable in the total proteome (all existing proteins) in blood. Here, we test the application of nascent proteomics as a novel approach for stroke biomarker discovery. METHODS - : The PBMC nascent proteome in human blood was determined by metabolic labeling of fresh PBMC cultures with azidohomoalanine (an azide-containing methionine surrogate), followed by mass spectrometry detection and quantification of azidohomoalanine-labeled proteins. The PBMC nascent and total proteomes were compared between patients with stroke and matched controls. RESULTS - : Both PBMC nascent and total proteomes showed differences between stroke patients and controls. Results of hierarchical clustering analysis of proteomic data revealed greater changes in the nascent than in the total PBMC proteomes, supporting the usefulness of the PBMC nascent proteome as a novel source of stroke biomarkers. CONCLUSIONS - : Nascent proteomes in PBMC can be a novel source for biomarker discovery in human stroke.

Original languageEnglish (US)
Pages (from-to)1177-1179
Number of pages3
JournalStroke
Volume45
Issue number4
DOIs
StatePublished - 2014

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Proteome
Blood Cells
Biomarkers
Stroke
Proteomics
Azides
Methionine
Cluster Analysis
Blood Proteins
Mass Spectrometry
Proteins
Cell Culture Techniques

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Advanced and Specialized Nursing

Cite this

Nascent proteomes in peripheral blood mononuclear cells as a novel source for biomarker discovery in human stroke. / Bian, Fang; Simon, Roger P.; Li, Yun; David, Larry; Wainwright, Jolita; Hall, Casey L.; Frankel, Michael; Zhou, An.

In: Stroke, Vol. 45, No. 4, 2014, p. 1177-1179.

Research output: Contribution to journalArticle

Bian, F, Simon, RP, Li, Y, David, L, Wainwright, J, Hall, CL, Frankel, M & Zhou, A 2014, 'Nascent proteomes in peripheral blood mononuclear cells as a novel source for biomarker discovery in human stroke', Stroke, vol. 45, no. 4, pp. 1177-1179. https://doi.org/10.1161/STROKEAHA.113.004576
Bian, Fang ; Simon, Roger P. ; Li, Yun ; David, Larry ; Wainwright, Jolita ; Hall, Casey L. ; Frankel, Michael ; Zhou, An. / Nascent proteomes in peripheral blood mononuclear cells as a novel source for biomarker discovery in human stroke. In: Stroke. 2014 ; Vol. 45, No. 4. pp. 1177-1179.
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AU - Hall, Casey L.

AU - Frankel, Michael

AU - Zhou, An

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AB - BACKGROUND AND PURPOSE - : The proteome of newly synthesized proteins (nascent proteome) in peripheral blood mononuclear cells (PBMCs) can be a novel source of stroke biomarkers. Changes in the PBMC nascent proteome after stroke reflect the dynamic response-in-action not detectable in the total proteome (all existing proteins) in blood. Here, we test the application of nascent proteomics as a novel approach for stroke biomarker discovery. METHODS - : The PBMC nascent proteome in human blood was determined by metabolic labeling of fresh PBMC cultures with azidohomoalanine (an azide-containing methionine surrogate), followed by mass spectrometry detection and quantification of azidohomoalanine-labeled proteins. The PBMC nascent and total proteomes were compared between patients with stroke and matched controls. RESULTS - : Both PBMC nascent and total proteomes showed differences between stroke patients and controls. Results of hierarchical clustering analysis of proteomic data revealed greater changes in the nascent than in the total PBMC proteomes, supporting the usefulness of the PBMC nascent proteome as a novel source of stroke biomarkers. CONCLUSIONS - : Nascent proteomes in PBMC can be a novel source for biomarker discovery in human stroke.

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