Nasal associated lymphoid tissue & M cells, a window to persistent streptococcal infections

P. Patrick Cleary, Yugen Zhang, Haesun Park

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background & objectives: Intranasal infection of mice has been used as a model of streptococcal pharyngitis, as nasal associated lymphoid tissue (NALT) in this animal is structurally and functionally analogous to human tonsils. The present study was carried out to determine whether group A streptococci preferentially colonized or invaded NALT. Methods: Lux+ strain Sp3 was created and exponential phase bacteria were introduced intranasally into BALB/C female mice, total photon emission from selected areas was quantified, sections of NALT tissues were used for immunofluorescent staining and M cell staining. Results: Intranasal infection of mice with bioluminescent group A streptococci or unlabeled streptococci demonstrated that NALT was a primary target of this pathogen. Streptococci readily gained access to the blood stream from this site of infection. Immunofluorescence microscopy studies showed that M cells, dispersed along the mucosal epithelium adjacent to NALT, were preferentially infected and likely to provide the window through which streptococci reached the underlying tissue. Interpretation & conclusion: The present study suggests that this upper respiratory Gram positive pathogen uses a mechanism similar to that of enteric pathogens in the intestine to gain access to underlying tissue, lymphatics and blood.

Original languageEnglish (US)
Pages (from-to)57-60
Number of pages4
JournalIndian Journal of Medical Research, Supplement
Volume119
Issue numberMAY
StatePublished - 2004
Externally publishedYes

Fingerprint

Streptococcal Infections
Lymphoid Tissue
Nose
Streptococcus
Tissue
Pathogens
Infection
Inbred BALB C Mouse
Staining and Labeling
Blood
Pharyngitis
Palatine Tonsil
Photons
Fluorescence Microscopy
Intestines
Epithelium
Bacteria
Microscopic examination
Animals

Keywords

  • Group A streptococcus
  • Invasion
  • M cells
  • Mouse model
  • Spesis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Nasal associated lymphoid tissue & M cells, a window to persistent streptococcal infections. / Cleary, P. Patrick; Zhang, Yugen; Park, Haesun.

In: Indian Journal of Medical Research, Supplement, Vol. 119, No. MAY, 2004, p. 57-60.

Research output: Contribution to journalArticle

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