Nanoplasmonic biomolecular ruler with a 2 NM spatial resolution for probing restricted digestion of nucleic acids

Gang L. Liu, Fanqing Chen, Daniele Gerion, Yadong Yin, A. Paul Alivisatos, Joe Gray, Luke P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

One of the major challenges of quantitative biochemistry and molecular biology is to monitor enzymatic activity within a femtoliter volume in real time. We have constructed a novel nanoscale plasmonic probe-based molecular ruler, which can perform label-free, real-time, and sensitive monitoring of DNA footprint or length during nuclease enzymatic reactions occurring around single nanoplasmonic biomolecular ruler. The nanoplasmonic molecular ruler was created by tethering specifically-designed double-stranded DNA to single Au nanoparticles. DNA length differences of as little as 2 nm (6 base pairs) after nuclease digestion are differentiated by the corresponding plasmon resonance shifts of the Au-DNA nanoconjugate.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages1513-1515
Number of pages3
Volume1
ISBN (Print)0974361119, 9780974361116
Publication statusPublished - 2005
Externally publishedYes
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

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Keywords

  • Nanoparticles
  • Nuclease
  • Nucleic acids
  • Plasmon resonance

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

Cite this

Liu, G. L., Chen, F., Gerion, D., Yin, Y., Paul Alivisatos, A., Gray, J., & Lee, L. P. (2005). Nanoplasmonic biomolecular ruler with a 2 NM spatial resolution for probing restricted digestion of nucleic acids. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (Vol. 1, pp. 1513-1515). Transducer Research Foundation.