Nanoplasmonic biomolecular ruler with a 2 NM spatial resolution for probing restricted digestion of nucleic acids

Gang L. Liu, Fanqing Chen, Daniele Gerion, Yadong Yin, A. Paul Alivisatos, Joe W. Gray, Luke P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

One of the major challenges of quantitative biochemistry and molecular biology is to monitor enzymatic activity within a femtoliter volume in real time. We have constructed a novel nanoscale plasmonic probe-based molecular ruler, which can perform label-free, real-time, and sensitive monitoring of DNA footprint or length during nuclease enzymatic reactions occurring around single nanoplasmonic biomolecular ruler. The nanoplasmonic molecular ruler was created by tethering specifically-designed double-stranded DNA to single Au nanoparticles. DNA length differences of as little as 2 nm (6 base pairs) after nuclease digestion are differentiated by the corresponding plasmon resonance shifts of the Au-DNA nanoconjugate.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages1513-1515
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - Jan 1 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Keywords

  • Nanoparticles
  • Nuclease
  • Nucleic acids
  • Plasmon resonance

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

Fingerprint Dive into the research topics of 'Nanoplasmonic biomolecular ruler with a 2 NM spatial resolution for probing restricted digestion of nucleic acids'. Together they form a unique fingerprint.

  • Cite this

    Liu, G. L., Chen, F., Gerion, D., Yin, Y., Paul Alivisatos, A., Gray, J. W., & Lee, L. P. (2005). Nanoplasmonic biomolecular ruler with a 2 NM spatial resolution for probing restricted digestion of nucleic acids. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1513-1515). (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1). Transducer Research Foundation.