N-methyl-d-aspartate (NMDA) receptors are inactivated by trypsin

Charles Allen, Robert Brady, John Swann, Nobuaki Hori, David O. Carpenter

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Acute isolation of hippocampal CA3 pyramidal cells using trypsin produces neurons which respond to kainate and quisqualate but not N-methyl-d-aspartate (NMDA). Incubation of 6- to 12-day-old cultured hippocampal neurons or slices of pyriform cortex with trypsin irreversibly removes the NMDA responses normally present without significant effect on responses to kainate or quisqualate. These data indicate that the NMDA receptor has a trypsin-sensitive component which is necessary for agonist recognition or ion channel activation.

Original languageEnglish (US)
Pages (from-to)147-150
Number of pages4
JournalBrain Research
Volume458
Issue number1
DOIs
StatePublished - Aug 16 1988
Externally publishedYes

Fingerprint

Trypsin
Quisqualic Acid
Kainic Acid
Aspartic Acid
Neurons
Pyramidal Cells
Ion Channels
aspartic acid receptor
Piriform Cortex

Keywords

  • Hippocampal neuron
  • Kainate
  • N-methyl-d-aspartate
  • Pyriform cortex
  • Quisqualate
  • Trypsin

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

N-methyl-d-aspartate (NMDA) receptors are inactivated by trypsin. / Allen, Charles; Brady, Robert; Swann, John; Hori, Nobuaki; Carpenter, David O.

In: Brain Research, Vol. 458, No. 1, 16.08.1988, p. 147-150.

Research output: Contribution to journalArticle

Allen, C, Brady, R, Swann, J, Hori, N & Carpenter, DO 1988, 'N-methyl-d-aspartate (NMDA) receptors are inactivated by trypsin', Brain Research, vol. 458, no. 1, pp. 147-150. https://doi.org/10.1016/0006-8993(88)90507-0
Allen, Charles ; Brady, Robert ; Swann, John ; Hori, Nobuaki ; Carpenter, David O. / N-methyl-d-aspartate (NMDA) receptors are inactivated by trypsin. In: Brain Research. 1988 ; Vol. 458, No. 1. pp. 147-150.
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