N-methyl-d-aspartate (NMDA) receptors are inactivated by trypsin

Charles N. Allen; Robert Brady; John Swann; Nobuaki Hori; David O. Carpenter

doi:10.1016/0006-8993(88)90507-0

N-methyl-d-aspartate (NMDA) receptors are inactivated by trypsin

Charles N. Allen, Robert Brady, John Swann, Nobuaki Hori, David O. Carpenter

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Acute isolation of hippocampal CA3 pyramidal cells using trypsin produces neurons which respond to kainate and quisqualate but not N-methyl-d-aspartate (NMDA). Incubation of 6- to 12-day-old cultured hippocampal neurons or slices of pyriform cortex with trypsin irreversibly removes the NMDA responses normally present without significant effect on responses to kainate or quisqualate. These data indicate that the NMDA receptor has a trypsin-sensitive component which is necessary for agonist recognition or ion channel activation.

Original language	English (US)
Pages (from-to)	147-150
Number of pages	4
Journal	Brain research
Volume	458
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(88)90507-0
State	Published - Aug 16 1988
Externally published	Yes

Keywords

Hippocampal neuron
Kainate
N-methyl-d-aspartate
Pyriform cortex
Quisqualate
Trypsin

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(88)90507-0

Cite this

@article{328e9590923942df9eaab68fbacaab0f,

title = "N-methyl-d-aspartate (NMDA) receptors are inactivated by trypsin",

abstract = "Acute isolation of hippocampal CA3 pyramidal cells using trypsin produces neurons which respond to kainate and quisqualate but not N-methyl-d-aspartate (NMDA). Incubation of 6- to 12-day-old cultured hippocampal neurons or slices of pyriform cortex with trypsin irreversibly removes the NMDA responses normally present without significant effect on responses to kainate or quisqualate. These data indicate that the NMDA receptor has a trypsin-sensitive component which is necessary for agonist recognition or ion channel activation.",

keywords = "Hippocampal neuron, Kainate, N-methyl-d-aspartate, Pyriform cortex, Quisqualate, Trypsin",

author = "Allen, {Charles N.} and Robert Brady and John Swann and Nobuaki Hori and Carpenter, {David O.}",

note = "Funding Information: We thank Dr. Gary Banker, who was supported by NIH Grant NS 17112 for supplying the cultured hippocampal neurons. Charlene McAuffle provided excellent secretarial assistance. This work was supported by NIH-NINCDS grants to R.J.B. (NS-23071), J.W.S, (NS-18309) and D,O.C. (NS-23807).",

year = "1988",

month = aug,

day = "16",

doi = "10.1016/0006-8993(88)90507-0",

language = "English (US)",

volume = "458",

pages = "147--150",

journal = "Brain research",

issn = "0006-8993",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - N-methyl-d-aspartate (NMDA) receptors are inactivated by trypsin

AU - Allen, Charles N.

AU - Brady, Robert

AU - Swann, John

AU - Hori, Nobuaki

AU - Carpenter, David O.

N1 - Funding Information: We thank Dr. Gary Banker, who was supported by NIH Grant NS 17112 for supplying the cultured hippocampal neurons. Charlene McAuffle provided excellent secretarial assistance. This work was supported by NIH-NINCDS grants to R.J.B. (NS-23071), J.W.S, (NS-18309) and D,O.C. (NS-23807).

PY - 1988/8/16

Y1 - 1988/8/16

N2 - Acute isolation of hippocampal CA3 pyramidal cells using trypsin produces neurons which respond to kainate and quisqualate but not N-methyl-d-aspartate (NMDA). Incubation of 6- to 12-day-old cultured hippocampal neurons or slices of pyriform cortex with trypsin irreversibly removes the NMDA responses normally present without significant effect on responses to kainate or quisqualate. These data indicate that the NMDA receptor has a trypsin-sensitive component which is necessary for agonist recognition or ion channel activation.

AB - Acute isolation of hippocampal CA3 pyramidal cells using trypsin produces neurons which respond to kainate and quisqualate but not N-methyl-d-aspartate (NMDA). Incubation of 6- to 12-day-old cultured hippocampal neurons or slices of pyriform cortex with trypsin irreversibly removes the NMDA responses normally present without significant effect on responses to kainate or quisqualate. These data indicate that the NMDA receptor has a trypsin-sensitive component which is necessary for agonist recognition or ion channel activation.

KW - Hippocampal neuron

KW - Kainate

KW - N-methyl-d-aspartate

KW - Pyriform cortex

KW - Quisqualate

KW - Trypsin

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DO - 10.1016/0006-8993(88)90507-0

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AN - SCOPUS:0023707480

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VL - 458

SP - 147

EP - 150

JO - Brain research

JF - Brain research

IS - 1

ER -

N-methyl-d-aspartate (NMDA) receptors are inactivated by trypsin

Abstract

Keywords

ASJC Scopus subject areas

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