Myristylation of pp60(c-src) is not required for complex formation with polyomavirus middle-T antigen

A. Wyss, S. Kaech, K. Ballmer-Hofer

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6 Scopus citations

Abstract

Middle-T antigen (middle-T), the transforming gene product of polyomavirus, associates with several cellular tyrosine kinases, such as pp60(c-src). Complex formation leads to kinase activation and is essential for cell transformation. Middle-T-associated as well as uncomplexed pp60(c-src) is predominantly found in the plasma membrane. We transfected mouse 3T3 fibroblasts with a mutated c-src gene (2Ac-src), allowing the expression of a protein containing alanine instead of glycine in position 2 of the primary translation product. Contrary to the wild-type c-src gene product, pp60(c-src(2A)) was not myristylated and accumulated in the cytoplasm instead of being transferred to cellular membranes. The mutant protein was able to associate with middle-T and was activated similarly to the wild-type c-src gene product. Both wild-type and 2A mutant protein were membrane associated upon complex formation with middle-T. This finding suggests that the putative carboxy-terminal membrane anchor sequence of middle-T is sufficient to hold middle-T-associated pp60(c-src(2A)) in the plasma membrane.

Original languageEnglish (US)
Pages (from-to)5163-5166
Number of pages4
JournalJournal of virology
Volume64
Issue number10
StatePublished - Jan 1 1990

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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