MyosinIIa contractility is required for maintenance of platelet structure during spreading on collagen and contributes to thrombus stability

Simon D.J. Calaminus, J. M. Auger, O. J.T. Mccarty, M. J.O. Wakelam, L. M. Machesky, S. P. Watson

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Background: MyosinIIs are adenosine triphosphate-driven molecular motors that form part of a cell's contractile machinery. They are activated by phosphorylation of their light chains, by either activation of myosin light chain (MLC) kinase or inhibition of MLC phosphatase via Rho kinase (ROCK). MyosinIIa phosphorylation underlies platelet rounding and stress fiber formation. Objective: To identify the functional significance of myosinIIa in platelet spreading and thrombus formation on collagen using inhibitors of ROCK (Y27632) and myosinII (blebbistatin). Results: Stress fiber formation on collagen is inhibited by both Y27632 and blebbistatin. A substantial proportion of spread platelets generate internal holes or splits on collagen, presumably because of a reduction in contractile strength. Platelet integrity, however, is maintained. In an in vitro model, thrombus embolization on collagen is increased in the presence of Y27632 and blebbistatin at intermediate shear, leading to a reduction in platelet aggregate growth. Moreover, Y27632 causes a marked reduction in thrombus formation in an in vivo laser-injury model. Conclusions: MyosinIIa contractility is required for maintenance of platelet structure during spreading on collagen and contributes to thrombus stability.

Original languageEnglish (US)
Pages (from-to)2136-2145
Number of pages10
JournalJournal of Thrombosis and Haemostasis
Volume5
Issue number10
DOIs
StatePublished - Oct 1 2007

Keywords

  • Arterial thrombosis
  • Cell signaling
  • MyosinII
  • Platelet morphology
  • Rho kinase
  • RhoA

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'MyosinIIa contractility is required for maintenance of platelet structure during spreading on collagen and contributes to thrombus stability'. Together they form a unique fingerprint.

  • Cite this