Myosin heavy chain composition in rat laryngeal muscles after denervation

Akihiro Shiotani, Paul Flint

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Objectives: The effects of denervation on myosin heavy chain (MHC) expression in specific laryngeal muscles are characterized using gel electrophoresis. Observed temporal changes in MHC composition will then be used as a biologic marker in studies designed to develop strategies for laryngeal reinnervation and gene therapy. Study Design: Animal study using an adult rat model for laryngeal paralysis. Methods: In anesthetized rats the left recurrent and superior laryngeal nerve were divided. Animals were survived for 7, 14, 28, 90, and 180 days. Animals were euthanized and the thyroarytenoid (TA), vocalis (VOC), posterior cricoarytenoid (PCA), lateral cricoarytenoid (LCA), and cricothyroid (CT) muscle excised. Each muscle was processed for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE) and densitometric measurements were obtained to determine composition of MHC fiber types. Results: The changes in relative MHC composition are described for each specific laryngeal muscle. In general, a decrease in type IIB and an increase in IIA and IIX are seen after denervation. Expression of IIL in the denervated condition is variable and the relative change in type I is minimal. Conclusion: This study supports previous work using rat soleus muscle in which IIA/IIX expression is favored in conditions with decreased neuromuscular activity, and conversely, IIB expression is activity dependent. Expression of type I appears to be independent of neural activity. Further study will be undertaken to quantify expression of MHC components and to study factors modulating expression.

Original languageEnglish (US)
Pages (from-to)1225-1229
Number of pages5
JournalLaryngoscope
Volume108
Issue number8 I
DOIs
StatePublished - Aug 1998
Externally publishedYes

Fingerprint

Muscle Denervation
Laryngeal Muscles
Myosin Heavy Chains
Denervation
Laryngeal Nerves
Recurrent Laryngeal Nerve
Vocal Cord Paralysis
Sodium Dodecyl Sulfate
Genetic Therapy
Electrophoresis
Polyacrylamide Gel Electrophoresis
Skeletal Muscle
Biomarkers
Gels
Muscles

Keywords

  • Denervation
  • Myosin heavy chain
  • Rat larynx
  • Recurrent laryngeal nerve
  • SDS-PAGE

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Myosin heavy chain composition in rat laryngeal muscles after denervation. / Shiotani, Akihiro; Flint, Paul.

In: Laryngoscope, Vol. 108, No. 8 I, 08.1998, p. 1225-1229.

Research output: Contribution to journalArticle

Shiotani, Akihiro ; Flint, Paul. / Myosin heavy chain composition in rat laryngeal muscles after denervation. In: Laryngoscope. 1998 ; Vol. 108, No. 8 I. pp. 1225-1229.
@article{a3df2fc43f3b4bbc866ea23423016cbf,
title = "Myosin heavy chain composition in rat laryngeal muscles after denervation",
abstract = "Objectives: The effects of denervation on myosin heavy chain (MHC) expression in specific laryngeal muscles are characterized using gel electrophoresis. Observed temporal changes in MHC composition will then be used as a biologic marker in studies designed to develop strategies for laryngeal reinnervation and gene therapy. Study Design: Animal study using an adult rat model for laryngeal paralysis. Methods: In anesthetized rats the left recurrent and superior laryngeal nerve were divided. Animals were survived for 7, 14, 28, 90, and 180 days. Animals were euthanized and the thyroarytenoid (TA), vocalis (VOC), posterior cricoarytenoid (PCA), lateral cricoarytenoid (LCA), and cricothyroid (CT) muscle excised. Each muscle was processed for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE) and densitometric measurements were obtained to determine composition of MHC fiber types. Results: The changes in relative MHC composition are described for each specific laryngeal muscle. In general, a decrease in type IIB and an increase in IIA and IIX are seen after denervation. Expression of IIL in the denervated condition is variable and the relative change in type I is minimal. Conclusion: This study supports previous work using rat soleus muscle in which IIA/IIX expression is favored in conditions with decreased neuromuscular activity, and conversely, IIB expression is activity dependent. Expression of type I appears to be independent of neural activity. Further study will be undertaken to quantify expression of MHC components and to study factors modulating expression.",
keywords = "Denervation, Myosin heavy chain, Rat larynx, Recurrent laryngeal nerve, SDS-PAGE",
author = "Akihiro Shiotani and Paul Flint",
year = "1998",
month = "8",
doi = "10.1097/00005537-199808000-00023",
language = "English (US)",
volume = "108",
pages = "1225--1229",
journal = "Laryngoscope",
issn = "0023-852X",
publisher = "John Wiley and Sons Inc.",
number = "8 I",

}

TY - JOUR

T1 - Myosin heavy chain composition in rat laryngeal muscles after denervation

AU - Shiotani, Akihiro

AU - Flint, Paul

PY - 1998/8

Y1 - 1998/8

N2 - Objectives: The effects of denervation on myosin heavy chain (MHC) expression in specific laryngeal muscles are characterized using gel electrophoresis. Observed temporal changes in MHC composition will then be used as a biologic marker in studies designed to develop strategies for laryngeal reinnervation and gene therapy. Study Design: Animal study using an adult rat model for laryngeal paralysis. Methods: In anesthetized rats the left recurrent and superior laryngeal nerve were divided. Animals were survived for 7, 14, 28, 90, and 180 days. Animals were euthanized and the thyroarytenoid (TA), vocalis (VOC), posterior cricoarytenoid (PCA), lateral cricoarytenoid (LCA), and cricothyroid (CT) muscle excised. Each muscle was processed for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE) and densitometric measurements were obtained to determine composition of MHC fiber types. Results: The changes in relative MHC composition are described for each specific laryngeal muscle. In general, a decrease in type IIB and an increase in IIA and IIX are seen after denervation. Expression of IIL in the denervated condition is variable and the relative change in type I is minimal. Conclusion: This study supports previous work using rat soleus muscle in which IIA/IIX expression is favored in conditions with decreased neuromuscular activity, and conversely, IIB expression is activity dependent. Expression of type I appears to be independent of neural activity. Further study will be undertaken to quantify expression of MHC components and to study factors modulating expression.

AB - Objectives: The effects of denervation on myosin heavy chain (MHC) expression in specific laryngeal muscles are characterized using gel electrophoresis. Observed temporal changes in MHC composition will then be used as a biologic marker in studies designed to develop strategies for laryngeal reinnervation and gene therapy. Study Design: Animal study using an adult rat model for laryngeal paralysis. Methods: In anesthetized rats the left recurrent and superior laryngeal nerve were divided. Animals were survived for 7, 14, 28, 90, and 180 days. Animals were euthanized and the thyroarytenoid (TA), vocalis (VOC), posterior cricoarytenoid (PCA), lateral cricoarytenoid (LCA), and cricothyroid (CT) muscle excised. Each muscle was processed for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE) and densitometric measurements were obtained to determine composition of MHC fiber types. Results: The changes in relative MHC composition are described for each specific laryngeal muscle. In general, a decrease in type IIB and an increase in IIA and IIX are seen after denervation. Expression of IIL in the denervated condition is variable and the relative change in type I is minimal. Conclusion: This study supports previous work using rat soleus muscle in which IIA/IIX expression is favored in conditions with decreased neuromuscular activity, and conversely, IIB expression is activity dependent. Expression of type I appears to be independent of neural activity. Further study will be undertaken to quantify expression of MHC components and to study factors modulating expression.

KW - Denervation

KW - Myosin heavy chain

KW - Rat larynx

KW - Recurrent laryngeal nerve

KW - SDS-PAGE

UR - http://www.scopus.com/inward/record.url?scp=0031858833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031858833&partnerID=8YFLogxK

U2 - 10.1097/00005537-199808000-00023

DO - 10.1097/00005537-199808000-00023

M3 - Article

C2 - 9707248

AN - SCOPUS:0031858833

VL - 108

SP - 1225

EP - 1229

JO - Laryngoscope

JF - Laryngoscope

SN - 0023-852X

IS - 8 I

ER -