Myosin heavy chain composition in human laryngeal muscles

Akihiro Shiotani, William H. Westra, Paul Flint

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Objectives: Myosin heavy chain (MHC) composition of human thyroarytenoid (TA), lateral cricoarytenoid (LCA), interarytenoid (LA), vocalis, posterior cricoarytenoid (PCA), and cricothyroid muscles were examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western bolt techniques. The presence of superfast MHC was also assessed using antibodies directed against the extraocular MHC. Study Design: MHC protein was analyzed using fresh human laryngeal muscles. Methods: Laryngeal muscles excised from cadavers were processed for SDS-PAGE. The composition of MHC isoforms was determined by densitometry. Western blot was carried out to identify specific bands. Results: MHC types IIA and lib are the predominant MHC components in human laryngeal muscles. The adductor muscles - TA, LCA, and IA - have a higher percentage of type IIB MHC and a lower percentage of type I when compared with the abductor - PCA. The rank file order for type IIb MHC composition (TA>LCA≥IA>PCA) is the same in all specimens. A band migrating between type IIA and type I was observed in several specimens. Although similar to type IIL in rats, this atypical band did not react with antiextraocular MHC antibody on Western blot. Conclusion: Characterization of laryngeal muscles determined by the composition of MHC is correlated with function and neural input. Human laryngeal muscle is characterized by a predominance of fast-type MHCs in laryngeal closing muscle and mixed fast- slow type MHCs in respiratory and phonatory muscle groups. Although an atypical myosin band similar to type IIL (superfast) MHC in rat was identified, it did not react with anti-extraocular MHC antibody.

Original languageEnglish (US)
Pages (from-to)1521-1524
Number of pages4
JournalLaryngoscope
Volume109
Issue number9
DOIs
StatePublished - Sep 1999
Externally publishedYes

Fingerprint

Laryngeal Muscles
Myosin Heavy Chains
Sodium Dodecyl Sulfate
Antibodies
Polyacrylamide Gel Electrophoresis
Western Blotting
Respiratory Muscles
Densitometry
Myosins
Cadaver

Keywords

  • Human laryngeal muscle
  • Myosin heavy chain
  • Polyacrylamide gel electrophoresis
  • Sodium dodecyl sulfate
  • Western blot

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Myosin heavy chain composition in human laryngeal muscles. / Shiotani, Akihiro; Westra, William H.; Flint, Paul.

In: Laryngoscope, Vol. 109, No. 9, 09.1999, p. 1521-1524.

Research output: Contribution to journalArticle

Shiotani, Akihiro ; Westra, William H. ; Flint, Paul. / Myosin heavy chain composition in human laryngeal muscles. In: Laryngoscope. 1999 ; Vol. 109, No. 9. pp. 1521-1524.
@article{6f09e6798ded4b97a6514ee1ea905a70,
title = "Myosin heavy chain composition in human laryngeal muscles",
abstract = "Objectives: Myosin heavy chain (MHC) composition of human thyroarytenoid (TA), lateral cricoarytenoid (LCA), interarytenoid (LA), vocalis, posterior cricoarytenoid (PCA), and cricothyroid muscles were examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western bolt techniques. The presence of superfast MHC was also assessed using antibodies directed against the extraocular MHC. Study Design: MHC protein was analyzed using fresh human laryngeal muscles. Methods: Laryngeal muscles excised from cadavers were processed for SDS-PAGE. The composition of MHC isoforms was determined by densitometry. Western blot was carried out to identify specific bands. Results: MHC types IIA and lib are the predominant MHC components in human laryngeal muscles. The adductor muscles - TA, LCA, and IA - have a higher percentage of type IIB MHC and a lower percentage of type I when compared with the abductor - PCA. The rank file order for type IIb MHC composition (TA>LCA≥IA>PCA) is the same in all specimens. A band migrating between type IIA and type I was observed in several specimens. Although similar to type IIL in rats, this atypical band did not react with antiextraocular MHC antibody on Western blot. Conclusion: Characterization of laryngeal muscles determined by the composition of MHC is correlated with function and neural input. Human laryngeal muscle is characterized by a predominance of fast-type MHCs in laryngeal closing muscle and mixed fast- slow type MHCs in respiratory and phonatory muscle groups. Although an atypical myosin band similar to type IIL (superfast) MHC in rat was identified, it did not react with anti-extraocular MHC antibody.",
keywords = "Human laryngeal muscle, Myosin heavy chain, Polyacrylamide gel electrophoresis, Sodium dodecyl sulfate, Western blot",
author = "Akihiro Shiotani and Westra, {William H.} and Paul Flint",
year = "1999",
month = "9",
doi = "10.1097/00005537-199909000-00030",
language = "English (US)",
volume = "109",
pages = "1521--1524",
journal = "Laryngoscope",
issn = "0023-852X",
publisher = "John Wiley and Sons Inc.",
number = "9",

}

TY - JOUR

T1 - Myosin heavy chain composition in human laryngeal muscles

AU - Shiotani, Akihiro

AU - Westra, William H.

AU - Flint, Paul

PY - 1999/9

Y1 - 1999/9

N2 - Objectives: Myosin heavy chain (MHC) composition of human thyroarytenoid (TA), lateral cricoarytenoid (LCA), interarytenoid (LA), vocalis, posterior cricoarytenoid (PCA), and cricothyroid muscles were examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western bolt techniques. The presence of superfast MHC was also assessed using antibodies directed against the extraocular MHC. Study Design: MHC protein was analyzed using fresh human laryngeal muscles. Methods: Laryngeal muscles excised from cadavers were processed for SDS-PAGE. The composition of MHC isoforms was determined by densitometry. Western blot was carried out to identify specific bands. Results: MHC types IIA and lib are the predominant MHC components in human laryngeal muscles. The adductor muscles - TA, LCA, and IA - have a higher percentage of type IIB MHC and a lower percentage of type I when compared with the abductor - PCA. The rank file order for type IIb MHC composition (TA>LCA≥IA>PCA) is the same in all specimens. A band migrating between type IIA and type I was observed in several specimens. Although similar to type IIL in rats, this atypical band did not react with antiextraocular MHC antibody on Western blot. Conclusion: Characterization of laryngeal muscles determined by the composition of MHC is correlated with function and neural input. Human laryngeal muscle is characterized by a predominance of fast-type MHCs in laryngeal closing muscle and mixed fast- slow type MHCs in respiratory and phonatory muscle groups. Although an atypical myosin band similar to type IIL (superfast) MHC in rat was identified, it did not react with anti-extraocular MHC antibody.

AB - Objectives: Myosin heavy chain (MHC) composition of human thyroarytenoid (TA), lateral cricoarytenoid (LCA), interarytenoid (LA), vocalis, posterior cricoarytenoid (PCA), and cricothyroid muscles were examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western bolt techniques. The presence of superfast MHC was also assessed using antibodies directed against the extraocular MHC. Study Design: MHC protein was analyzed using fresh human laryngeal muscles. Methods: Laryngeal muscles excised from cadavers were processed for SDS-PAGE. The composition of MHC isoforms was determined by densitometry. Western blot was carried out to identify specific bands. Results: MHC types IIA and lib are the predominant MHC components in human laryngeal muscles. The adductor muscles - TA, LCA, and IA - have a higher percentage of type IIB MHC and a lower percentage of type I when compared with the abductor - PCA. The rank file order for type IIb MHC composition (TA>LCA≥IA>PCA) is the same in all specimens. A band migrating between type IIA and type I was observed in several specimens. Although similar to type IIL in rats, this atypical band did not react with antiextraocular MHC antibody on Western blot. Conclusion: Characterization of laryngeal muscles determined by the composition of MHC is correlated with function and neural input. Human laryngeal muscle is characterized by a predominance of fast-type MHCs in laryngeal closing muscle and mixed fast- slow type MHCs in respiratory and phonatory muscle groups. Although an atypical myosin band similar to type IIL (superfast) MHC in rat was identified, it did not react with anti-extraocular MHC antibody.

KW - Human laryngeal muscle

KW - Myosin heavy chain

KW - Polyacrylamide gel electrophoresis

KW - Sodium dodecyl sulfate

KW - Western blot

UR - http://www.scopus.com/inward/record.url?scp=0032887182&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032887182&partnerID=8YFLogxK

U2 - 10.1097/00005537-199909000-00030

DO - 10.1097/00005537-199909000-00030

M3 - Article

C2 - 10499066

AN - SCOPUS:0032887182

VL - 109

SP - 1521

EP - 1524

JO - Laryngoscope

JF - Laryngoscope

SN - 0023-852X

IS - 9

ER -