Myosin 1c participates in B cell cytoskeleton rearrangements, is recruited to the immunologic synapse, and contributes to antigen presentation

José L. Maravillas-Montero, Peter Barr-Gillespie, Genaro Patiño-López, Stephen Shaw, Leopoldo Santos-Argumedo

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Myosin 1c (Myo1c) is a member of the unconventional class I myosins of vertebrates, which directly link the plasma membrane with the microfilament cortical web. Although this molecular motor has been implicated in cell functions such as cytoskeleton organization, cell motility, nuclear transcription, and endocytosis, its role in hematopoietic cells is largely unknown. In this study, we show that Myo1c is abundantly expressed in murine B lymphocytes and is preferentially located at the plasma membrane, especially in peripheral processes such as microvilli.We observed that this motor concentrates at the growing membrane protrusions generated during B cell spreading and that it is actively recruited to the immune synapse. Interestingly, Myo1c was detected in lipid rafts of B cells and showed strong colocalization with MHC-II, particularly after cross-linking of these molecules. By transfection of a dominant negative formofMyo1c or specific siRNA,we also detected alterations in the spreading andAg-presenting ability of these cells. The data suggest that Myo1c is involved in the cytoskeleton dynamics and membrane protein anchoring or sorting in B lymphocytes.

Original languageEnglish (US)
Pages (from-to)3053-3063
Number of pages11
JournalJournal of Immunology
Volume187
Issue number6
DOIs
StatePublished - Sep 15 2011

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Antigen Presentation
Myosins
Cytoskeleton
Synapses
B-Lymphocytes
Myosin Type I
Cell Membrane
Microvilli
Endocytosis
Actin Cytoskeleton
Small Interfering RNA
Cell Movement
Transfection
Vertebrates
Membrane Proteins
Lipids
Membranes

ASJC Scopus subject areas

  • Immunology

Cite this

Myosin 1c participates in B cell cytoskeleton rearrangements, is recruited to the immunologic synapse, and contributes to antigen presentation. / Maravillas-Montero, José L.; Barr-Gillespie, Peter; Patiño-López, Genaro; Shaw, Stephen; Santos-Argumedo, Leopoldo.

In: Journal of Immunology, Vol. 187, No. 6, 15.09.2011, p. 3053-3063.

Research output: Contribution to journalArticle

Maravillas-Montero, José L. ; Barr-Gillespie, Peter ; Patiño-López, Genaro ; Shaw, Stephen ; Santos-Argumedo, Leopoldo. / Myosin 1c participates in B cell cytoskeleton rearrangements, is recruited to the immunologic synapse, and contributes to antigen presentation. In: Journal of Immunology. 2011 ; Vol. 187, No. 6. pp. 3053-3063.
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