Myometrial prostaglandin E2 synthetic enzyme mRNA expression: Spatial and temporal variations with pregnancy and labour

S. R. Sooranna, Peta Grigsby, N. Engineer, Z. Liang, K. Sun, Leslie Myatt, Mark R. Johnson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We have investigated the hypothesis that the expression of the enzymes involved in PGE2 synthesis in the human uterus is co-ordinated. We have studied (i) the mRNA expression of the enzymes involved in PGE2 synthesis [phospholipases (cPLA2 and sPLA2), prostaglandin H synthase (PGHS)-2 and PG E synthases (PGES-1 and -2)] and their relationship to the expression of inflammatory cytokines in samples of myometrium obtained from pregnant women undergoing caesarean section (LSCS) either before or after the onset of labour at or before term; and (ii) the effect of IL-1β, IL-6, TNF-α, PGE2 and stretch on PGE2 enzyme mRNA expression. We found that cPLA2, sPLA2 and PGHS-2 mRNA expression were greater in labour samples; cPLA2, sPLA2, PGHS-2, PGES-1 and -2 mRNA expression were greater in lower - than upper-segment samples; and there was no effect of gestational age. PGHS-2 mRNA levels correlated with those of PGES-1, cPLA2, IL-1β and IL-8; PGES-1 mRNA levels correlated with those of IL-1β, IL-8 and cPLA2. In primary cultures of uterine myocytes, cPLA2 mRNA expression was increased by IL-1β and IL-6; PGHS-2 mRNA expression was increased by IL-1β, PGE2 and stretch; and PGES-1 mRNA expression was increased by IL-1β only. These data show that labour is associated with increased expression of the enzymes involved in PGE2 synthesis and their expression is greater in the lower uterine segment. The presence of associations between the levels of PGE2 enzyme mRNA expression and the effects of IL-1β suggest that their expression is co-ordinated and that IL-1β is the responsible factor.

Original languageEnglish (US)
Pages (from-to)625-631
Number of pages7
JournalMolecular Human Reproduction
Volume12
Issue number10
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Dinoprostone
Interleukin-1
Pregnancy
Messenger RNA
Enzymes
Cyclooxygenase 2
Interleukin-8
Interleukin-6
Cytosolic Phospholipases A2
Labor Onset
Myometrium
Cesarean Section
Muscle Cells
Gestational Age
Uterus
Pregnant Women
Cytokines

Keywords

  • Cytokines
  • Labour
  • Prostaglandin synthesis
  • Stretch
  • Uterine smooth muscle cells

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Genetics
  • Developmental Biology
  • Embryology
  • Cell Biology

Cite this

Myometrial prostaglandin E2 synthetic enzyme mRNA expression : Spatial and temporal variations with pregnancy and labour. / Sooranna, S. R.; Grigsby, Peta; Engineer, N.; Liang, Z.; Sun, K.; Myatt, Leslie; Johnson, Mark R.

In: Molecular Human Reproduction, Vol. 12, No. 10, 2006, p. 625-631.

Research output: Contribution to journalArticle

Sooranna, S. R. ; Grigsby, Peta ; Engineer, N. ; Liang, Z. ; Sun, K. ; Myatt, Leslie ; Johnson, Mark R. / Myometrial prostaglandin E2 synthetic enzyme mRNA expression : Spatial and temporal variations with pregnancy and labour. In: Molecular Human Reproduction. 2006 ; Vol. 12, No. 10. pp. 625-631.
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AB - We have investigated the hypothesis that the expression of the enzymes involved in PGE2 synthesis in the human uterus is co-ordinated. We have studied (i) the mRNA expression of the enzymes involved in PGE2 synthesis [phospholipases (cPLA2 and sPLA2), prostaglandin H synthase (PGHS)-2 and PG E synthases (PGES-1 and -2)] and their relationship to the expression of inflammatory cytokines in samples of myometrium obtained from pregnant women undergoing caesarean section (LSCS) either before or after the onset of labour at or before term; and (ii) the effect of IL-1β, IL-6, TNF-α, PGE2 and stretch on PGE2 enzyme mRNA expression. We found that cPLA2, sPLA2 and PGHS-2 mRNA expression were greater in labour samples; cPLA2, sPLA2, PGHS-2, PGES-1 and -2 mRNA expression were greater in lower - than upper-segment samples; and there was no effect of gestational age. PGHS-2 mRNA levels correlated with those of PGES-1, cPLA2, IL-1β and IL-8; PGES-1 mRNA levels correlated with those of IL-1β, IL-8 and cPLA2. In primary cultures of uterine myocytes, cPLA2 mRNA expression was increased by IL-1β and IL-6; PGHS-2 mRNA expression was increased by IL-1β, PGE2 and stretch; and PGES-1 mRNA expression was increased by IL-1β only. These data show that labour is associated with increased expression of the enzymes involved in PGE2 synthesis and their expression is greater in the lower uterine segment. The presence of associations between the levels of PGE2 enzyme mRNA expression and the effects of IL-1β suggest that their expression is co-ordinated and that IL-1β is the responsible factor.

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