Myelomonocytic crisis with t(5;17) and a p53 mutation in a patient with chronic myelogenous leukemia

H. Nakagawa, H. Fujii, Hiroyuki Nakai, J. Inazawa, S. Misawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We report a 64-year-old Japanese man with chronic myelogenous leukemia (CML) who expired with myelomonocytic crisis. Cytogenetic analyses of chronic phase (CP) and accelerated phase (AP) cells revealed a Philadelphia chromosome and an isochromosome for the long arm of chromosome 17, i(17q). This karyotype was replaced by another karyotype in blast crisis (BC), resulting in near triploidy with t(5;17) (p15;p11) and loss of chromosome 17pter→p11. Interphase fluorescent in situ hybridization studies with a chromosome 17 specific alpha satellite DNA probe confirmed the presence of a clonal change in BC. In addition, single-strand conformation polymorphism analysis and PCR-direct sequencing of BC cells revealed a point mutation at codon 203 of the p53 gene, GTG to GAG (Val to Glu), and loss of the normal allele. In contrast, no alterations of the p53 gene were found in CP and AP cells. Therefore, progression of CML in this patient appeared to be related to loss of 17p, as well as a mutation in the p53 gene.

Original languageEnglish (US)
Pages (from-to)335-340
Number of pages6
JournalAmerican Journal of Hematology
Volume45
Issue number4
StatePublished - 1994
Externally publishedYes

Fingerprint

Blast Crisis
p53 Genes
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Chromosomes, Human, Pair 17
Karyotype
Mutation
Isochromosomes
Satellite DNA
Triploidy
Philadelphia Chromosome
Cytogenetic Analysis
Interphase
DNA Probes
Fluorescence In Situ Hybridization
Point Mutation
Codon
Chromosomes
Alleles
Polymerase Chain Reaction

Keywords

  • CML
  • myelomonocytic crisis
  • p53 gene
  • t(5;17)

ASJC Scopus subject areas

  • Hematology

Cite this

Myelomonocytic crisis with t(5;17) and a p53 mutation in a patient with chronic myelogenous leukemia. / Nakagawa, H.; Fujii, H.; Nakai, Hiroyuki; Inazawa, J.; Misawa, S.

In: American Journal of Hematology, Vol. 45, No. 4, 1994, p. 335-340.

Research output: Contribution to journalArticle

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