Myelination of axons within cytosine arabinoside treated mouse cerebellar explants by cultured rat oligodendrocytes

Fredrick J. Seil, Marilyn L. Johnson, Russell P. Saneto, Robert M. Herndon, Michele K. Mass

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Cell suspensions of cultured purified rat oligodendrocytes prepared by the differential substrate adhesion method were applied to neonatal mouse cerebellar explant cultures in which myelination and oligodendrocyte maturation had been irreversibly inhibited by exposure to cytosine arabinoside. Myelination of Purkinje cell axons within 92% of the host explants was observed 2-5 days after oligodendrocyte application. Ultrastructurally, mature oligodendrocytes and axons surrounded by compact myelin, as well as spherules of compact myelin membranes without axons, were present within the cerebellar explants. It is evident that cultured dissociated purified oligodendrocytes retain the ability to myelinate appropriate axons. Such oligodendrocytes may be hyperreactive with regard to myelin membrane formation, as suggested by the presence of spheres of compact myelin without axons.

Original languageEnglish (US)
Pages (from-to)111-117
Number of pages7
JournalBrain research
Volume503
Issue number1
DOIs
StatePublished - Nov 27 1989

Keywords

  • Cerebellar explant culture
  • Cytosine arabinoside
  • Myelin spherule
  • Myelin-like membrane
  • Myelination
  • Purified oligodendrocytes
  • Transplantation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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