Myelin membrane biogenesis by oligodendrocytes Developmental regulation of low molecular weight GTP-binding proteins

Lukas A. Huber, Dana Madison, Kai Simons, S. E. Pfeiffer

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Oligodendrocytes synthesize dramatic amounts of myelin membrane. We hypothesized that this requires unique aspects of vesicular trafficking. Specific stages of the oligodendrocyte lineage were assayed for low molecular weight GTP-binding proteins implicated in the regulation of vesicular transport pathway (two dimensional gel electrophoresis, [α-32P]GTP overlay). Consistent with the hypothesis, as oligodendrocytes differentiate from early progenitors to mature myelin-producing cells, ≥≥ 12 small GTP-binding proteins become up-regulated. Myelin membrane also has a complex pattern of GTP-binding proteins. Several of these proteins may be specific to oligodendrocytes, suggesting that oligodendrocytes may utilize cell-type specific GTP-binding proteins for biogenesis and maintenance of the myelin membrane.

Original languageEnglish (US)
Pages (from-to)273-278
Number of pages6
JournalFEBS Letters
Volume347
Issue number2-3
DOIs
StatePublished - Jun 27 1994
Externally publishedYes

Fingerprint

Oligodendroglia
Myelin Sheath
GTP-Binding Proteins
Molecular Weight
Molecular weight
Membranes
Guanosine Triphosphate
Electrophoresis
Electrophoresis, Gel, Two-Dimensional
Gels
Maintenance
Proteins

Keywords

  • GTP-binding protein
  • Myelin biogenesis
  • Oligodendrocyte
  • Rab protein
  • Vesicular trafficking

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Myelin membrane biogenesis by oligodendrocytes Developmental regulation of low molecular weight GTP-binding proteins. / Huber, Lukas A.; Madison, Dana; Simons, Kai; Pfeiffer, S. E.

In: FEBS Letters, Vol. 347, No. 2-3, 27.06.1994, p. 273-278.

Research output: Contribution to journalArticle

Huber, Lukas A. ; Madison, Dana ; Simons, Kai ; Pfeiffer, S. E. / Myelin membrane biogenesis by oligodendrocytes Developmental regulation of low molecular weight GTP-binding proteins. In: FEBS Letters. 1994 ; Vol. 347, No. 2-3. pp. 273-278.
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