TY - JOUR
T1 - Myelin changes induced by incubation of brain slices with serum
AU - Offner, Halina
AU - Konat, Gregory
N1 - Funding Information:
Acknowledgements--The authors thank Mrs. Joan Mel-lach for her excellent technical assistance. The study was supported by the Danish Multiple Sclerosis Society.
PY - 1983
Y1 - 1983
N2 - Forebrain and brain stem slices prepared from adult rats were incubated with pooled normal human serum. Following the incubation, the tissue was homogenized and the fraction floating on 0.32 M sucrose as well as two myelin subfractions (light and heavy) were isolated. Addition of serum into the incubation medium increased generation of the floating fraction by the cerebral slices. Changes in the myelin membrane were also observed. Thus, myelin isolated from forebrain slices revealed pronounced increase in the buoyant density of its particles and loss of basic protein. Furthermore, in spite of the intensive washing employed during the isolation procedure, some serum proteins were found firmly attached to the membraneous fractions. The demonstration of the myelin alterations in the living cerebral tissue exposed to serum during incubation may contribute to understanding the pathogenesis of multiple sclerosis.
AB - Forebrain and brain stem slices prepared from adult rats were incubated with pooled normal human serum. Following the incubation, the tissue was homogenized and the fraction floating on 0.32 M sucrose as well as two myelin subfractions (light and heavy) were isolated. Addition of serum into the incubation medium increased generation of the floating fraction by the cerebral slices. Changes in the myelin membrane were also observed. Thus, myelin isolated from forebrain slices revealed pronounced increase in the buoyant density of its particles and loss of basic protein. Furthermore, in spite of the intensive washing employed during the isolation procedure, some serum proteins were found firmly attached to the membraneous fractions. The demonstration of the myelin alterations in the living cerebral tissue exposed to serum during incubation may contribute to understanding the pathogenesis of multiple sclerosis.
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U2 - 10.1016/0197-0186(83)90007-4
DO - 10.1016/0197-0186(83)90007-4
M3 - Article
AN - SCOPUS:0020576813
SN - 0197-0186
VL - 5
SP - 45
EP - 49
JO - Neurochemistry International
JF - Neurochemistry International
IS - 1
ER -