Myelin changes induced by incubation of brain slices with serum

Halina Offner, Gregory Konat

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Forebrain and brain stem slices prepared from adult rats were incubated with pooled normal human serum. Following the incubation, the tissue was homogenized and the fraction floating on 0.32 M sucrose as well as two myelin subfractions (light and heavy) were isolated. Addition of serum into the incubation medium increased generation of the floating fraction by the cerebral slices. Changes in the myelin membrane were also observed. Thus, myelin isolated from forebrain slices revealed pronounced increase in the buoyant density of its particles and loss of basic protein. Furthermore, in spite of the intensive washing employed during the isolation procedure, some serum proteins were found firmly attached to the membraneous fractions. The demonstration of the myelin alterations in the living cerebral tissue exposed to serum during incubation may contribute to understanding the pathogenesis of multiple sclerosis.

Original languageEnglish (US)
Pages (from-to)45-49
Number of pages5
JournalNeurochemistry International
Volume5
Issue number1
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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