TY - JOUR
T1 - Mycobacterium grossiae sp. Nov., a rapidly growing, scotochromogenic species isolated from human clinical respiratory and blood culture specimens
AU - Paniz-Mondolfi, Alberto Enrique
AU - Greninger, Alexander L.
AU - Ladutko, Lynn
AU - Brown-Elliott, Barbara A.
AU - Vasireddy, Ravikiran
AU - Jakubiec, Wesley
AU - Vasireddy, Sruthi
AU - Wallace, Richard J.
AU - Simmon, Keith E.
AU - Dunn, Bruce E.
AU - Jackoway, Gary
AU - Vora, Surabhi B.
AU - Quinn, Kevin K.
AU - Qin, Xuan
AU - Campbell, Sheldon
N1 - Publisher Copyright:
© 2017 IUMS.
PY - 2017/11
Y1 - 2017/11
N2 - A previously undescribed, rapidly growing, scotochromogenic species of the genus Mycobacterium (represented by strains PB739T and GK) was isolated from two clinical sources–the sputum of a 76-year-old patient with severe chronic obstructive pulmonary disease, history of tuberculosis exposure and Mycobacterium avium complex isolated years prior; and the blood of a 15-year-old male with B-cell acute lymphoblastic leukaemia status post bone marrow transplant. The isolates grew as dark orange colonies at 25–37 °C after 5 days, sharing features in common with other closely related species. Analysis of the complete 16S rRNA gene sequence (1492 bp) of strain PB739T demonstrated that the isolate shared 98.8% relatedness with Mycobacterium wolinskyi. Partial 429 bp hsp65 and 744 bp rpoB region V sequence analyses revealed that the sequences of the novel isolate shared 94.8 and 92.1% similarity with those of Mycobacterium neoaurum and Mycobacterium aurum, respectively. Biochemical profiling, antimicrobial susceptibility testing, HPLC/gas-liquid chromatography analyses and multilocus sequence typing support the taxonomic status of these isolates (PB739T and GK) as representatives of a novel species. Both isolates were susceptible to the Clinical and Laboratory Standards Institute recommended antimicrobials for susceptibility testing of rapidly growing mycobacteria including amikacin, ciprofloxacin, moxifloxacin, doxycycline/ minocycline, imipenem, linezolid, clarithromycin and trimethropin/sulfamethoxazole. Both isolates PB739T and GK showed intermediate susceptibility to cefoxitin. We propose the name Mycobacterium grossiae sp. nov. for this novel species and have deposited the type strain in the DSMZ and CIP culture collections. The type strain is PB739T (=DSM 104744T=CIP 111318T).
AB - A previously undescribed, rapidly growing, scotochromogenic species of the genus Mycobacterium (represented by strains PB739T and GK) was isolated from two clinical sources–the sputum of a 76-year-old patient with severe chronic obstructive pulmonary disease, history of tuberculosis exposure and Mycobacterium avium complex isolated years prior; and the blood of a 15-year-old male with B-cell acute lymphoblastic leukaemia status post bone marrow transplant. The isolates grew as dark orange colonies at 25–37 °C after 5 days, sharing features in common with other closely related species. Analysis of the complete 16S rRNA gene sequence (1492 bp) of strain PB739T demonstrated that the isolate shared 98.8% relatedness with Mycobacterium wolinskyi. Partial 429 bp hsp65 and 744 bp rpoB region V sequence analyses revealed that the sequences of the novel isolate shared 94.8 and 92.1% similarity with those of Mycobacterium neoaurum and Mycobacterium aurum, respectively. Biochemical profiling, antimicrobial susceptibility testing, HPLC/gas-liquid chromatography analyses and multilocus sequence typing support the taxonomic status of these isolates (PB739T and GK) as representatives of a novel species. Both isolates were susceptible to the Clinical and Laboratory Standards Institute recommended antimicrobials for susceptibility testing of rapidly growing mycobacteria including amikacin, ciprofloxacin, moxifloxacin, doxycycline/ minocycline, imipenem, linezolid, clarithromycin and trimethropin/sulfamethoxazole. Both isolates PB739T and GK showed intermediate susceptibility to cefoxitin. We propose the name Mycobacterium grossiae sp. nov. for this novel species and have deposited the type strain in the DSMZ and CIP culture collections. The type strain is PB739T (=DSM 104744T=CIP 111318T).
KW - Bacterial WGS
KW - Mycobacterium
KW - Mycobacterium grossiae
KW - Phylogenomics
KW - Rapidly growing
KW - Respiratory
KW - Scotochromogenic
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U2 - 10.1099/ijsem.0.002216
DO - 10.1099/ijsem.0.002216
M3 - Article
C2 - 28984546
AN - SCOPUS:85034226313
SN - 1466-5026
VL - 67
SP - 4345
EP - 4351
JO - International journal of systematic and evolutionary microbiology
JF - International journal of systematic and evolutionary microbiology
IS - 11
ER -