Myc requires distinct E2F activities to induce S phase and apoptosis

Gustavo Leone, Rosalie Sears, Erich Huang, Rachel Rempel, Faison Nuckolls, Chi Hyun Park, Paloma Giangrande, Lizhao Wu, Harold I. Saavedra, Seth J. Field, Margaret A. Thompson, Haidi Yang, Yuko Fujiwara, Michael E. Greenberg, Stuart Orkin, Clay Smith, Joseph R. Nevins

Research output: Contribution to journalArticle

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Abstract

Previous work has shown that the Myc transcription factor induces transcription of the E2F1, E2F2, and E2F3 genes. Using primary mouse embryo fibroblasts deleted for individual E2F genes, we now show that Myc-induced S phase and apoptosis requires distinct E2F activities. The ability of Myc to induce S phase is impaired in the absence of either E2F2 or E2F3 but not E2F1 or E2F4. In contrast, the ability of Myc to induce apoptosis is markedly reduced in cells deleted for E2F1 but not E2F2 or E2F3. From this data, we propose that the induction of specific E2F activities is an essential component in the Myc pathways that control cell proliferation and cell fate decisions.

Original languageEnglish (US)
Pages (from-to)105-113
Number of pages9
JournalMolecular Cell
Volume8
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

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S Phase
Apoptosis
Genes
Transcription Factors
Embryonic Structures
Fibroblasts
Cell Proliferation

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Leone, G., Sears, R., Huang, E., Rempel, R., Nuckolls, F., Park, C. H., ... Nevins, J. R. (2001). Myc requires distinct E2F activities to induce S phase and apoptosis. Molecular Cell, 8(1), 105-113. https://doi.org/10.1016/S1097-2765(01)00275-1

Myc requires distinct E2F activities to induce S phase and apoptosis. / Leone, Gustavo; Sears, Rosalie; Huang, Erich; Rempel, Rachel; Nuckolls, Faison; Park, Chi Hyun; Giangrande, Paloma; Wu, Lizhao; Saavedra, Harold I.; Field, Seth J.; Thompson, Margaret A.; Yang, Haidi; Fujiwara, Yuko; Greenberg, Michael E.; Orkin, Stuart; Smith, Clay; Nevins, Joseph R.

In: Molecular Cell, Vol. 8, No. 1, 2001, p. 105-113.

Research output: Contribution to journalArticle

Leone, G, Sears, R, Huang, E, Rempel, R, Nuckolls, F, Park, CH, Giangrande, P, Wu, L, Saavedra, HI, Field, SJ, Thompson, MA, Yang, H, Fujiwara, Y, Greenberg, ME, Orkin, S, Smith, C & Nevins, JR 2001, 'Myc requires distinct E2F activities to induce S phase and apoptosis', Molecular Cell, vol. 8, no. 1, pp. 105-113. https://doi.org/10.1016/S1097-2765(01)00275-1
Leone, Gustavo ; Sears, Rosalie ; Huang, Erich ; Rempel, Rachel ; Nuckolls, Faison ; Park, Chi Hyun ; Giangrande, Paloma ; Wu, Lizhao ; Saavedra, Harold I. ; Field, Seth J. ; Thompson, Margaret A. ; Yang, Haidi ; Fujiwara, Yuko ; Greenberg, Michael E. ; Orkin, Stuart ; Smith, Clay ; Nevins, Joseph R. / Myc requires distinct E2F activities to induce S phase and apoptosis. In: Molecular Cell. 2001 ; Vol. 8, No. 1. pp. 105-113.
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AU - Leone, Gustavo

AU - Sears, Rosalie

AU - Huang, Erich

AU - Rempel, Rachel

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AU - Park, Chi Hyun

AU - Giangrande, Paloma

AU - Wu, Lizhao

AU - Saavedra, Harold I.

AU - Field, Seth J.

AU - Thompson, Margaret A.

AU - Yang, Haidi

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AU - Orkin, Stuart

AU - Smith, Clay

AU - Nevins, Joseph R.

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AB - Previous work has shown that the Myc transcription factor induces transcription of the E2F1, E2F2, and E2F3 genes. Using primary mouse embryo fibroblasts deleted for individual E2F genes, we now show that Myc-induced S phase and apoptosis requires distinct E2F activities. The ability of Myc to induce S phase is impaired in the absence of either E2F2 or E2F3 but not E2F1 or E2F4. In contrast, the ability of Myc to induce apoptosis is markedly reduced in cells deleted for E2F1 but not E2F2 or E2F3. From this data, we propose that the induction of specific E2F activities is an essential component in the Myc pathways that control cell proliferation and cell fate decisions.

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