Mutations resulting in resistance to polyene antibiotics decrease voltage-sensitive calcium channel activity in paramecium

Michael Forte, Todd Hennessey, Ching Kung

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this report, the isolation of Paramecium tetraurelia mutants resistant to the polyene antibiotics amphotericin B and filipin are described. These antibiotics are known to specifically interact with membrane sterols to produce a cytotoxic effect. Four mutants resistant to amphotericin B and two mutants resistant to filipin have been isolated. In each case, an individual mutant shows resistance to both amphotericin B and filipin. Genetic analysis indicates that all 6 mutations map to the same complementation group and are not allelic to any of the 3 pawn mutations or to either of the two "barium shy" mutations. The behavioral analysis suggests that these mutations cause an alteration of normal ion channel function. Direct electrophysiological analysis of one mutant indicates mutations to polyene antibiotic resistance cause a specific decrease in voltage-sensitive Ca2+ channel activity.

Original languageEnglish (US)
Pages (from-to)75-85
Number of pages11
JournalJournal of Neurogenetics
Volume3
Issue number2
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Paramecium
Polyenes
Calcium Channels
Filipin
Anti-Bacterial Agents
Amphotericin B
Mutation
Paramecium tetraurelia
Sterols
Barium
Microbial Drug Resistance
Ion Channels
Membranes

Keywords

  • Genetic dissection
  • Ion channel
  • Membrane sterol
  • Paramecium mutant
  • Polyene antibiotic

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Genetics
  • Developmental Biology
  • Neuroscience(all)

Cite this

Mutations resulting in resistance to polyene antibiotics decrease voltage-sensitive calcium channel activity in paramecium. / Forte, Michael; Hennessey, Todd; Kung, Ching.

In: Journal of Neurogenetics, Vol. 3, No. 2, 1986, p. 75-85.

Research output: Contribution to journalArticle

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