Mutations in the env gene of friend spleen focus-forming virus overcome Fv-2r-mediated resistance to friend virus-induced erythroleukemia

Manas K. Majumdar, Chung Lung Cho, Mary T. Fox, Kristine L. Eckner, Sue Kozak, David Kabat, Roy W. Geib

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Although Fv-2r homozygous mice are resistant to leukemias induced either by an erythropoietin-encoding virus or by wild-type Friend virus (FV) (M. E. Hoatlin, S. L. Kozak, F. Lilly, A. Chakraborti, C. A. Kozak, and D. Kabat, Proc. Natl. Acad. Sci. USA 87:9985-9989, 1990), they are susceptible to some variants of FV (R. A. Steeves, E. A. Mirand, A. Bulba, and P. J. Trudel, Int. J. Cancer 5:349-356, 1970; R. W. Geib, M. B. Seaward, M. L. Stevens, C.-L. Cho, and M. Majumdar, Virus Res. 14:161-174, 1989). To localize the virus gene involved in influencing the host range, we cloned and sequenced the env gene of the BB6 variant of FV (Steeves et al., Int. J. Cancer 5:349-356, 1970). In comparison with the wild-type env gene, the BB6 variant contains a 159-bp deletion that eliminates the membrane-proximal portion of the extracellular domain and 58 point mutations resulting in 13 amino acid changes. Substitution of the variant env gene for the wild-type env gene resulted in a recombinant virus that produced a Friend virus-like disease in Fv-2r homozygotes. Our results identify the spleen focus-forming virus env gene as the viral gene involved in this virus-host interaction. Additionally, they suggest that the product of the Fv-2r gene modifies the interaction between the spleen focus-forming virus envelope protein and the erythropoietin receptor.

Original languageEnglish (US)
Pages (from-to)3652-3660
Number of pages9
JournalJournal of Virology
Volume66
Issue number6
StatePublished - Jun 1992
Externally publishedYes

Fingerprint

Spleen Focus-Forming Viruses
Friend murine leukemia virus
env Genes
Leukemia, Erythroblastic, Acute
spleen
Viruses
mutation
viruses
Mutation
genes
Erythropoietin Receptors
Viral Envelope Proteins
Viral Genes
erythropoietin
Host Specificity
Homozygote
Virus Diseases
Erythropoietin
Point Mutation
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Majumdar, M. K., Cho, C. L., Fox, M. T., Eckner, K. L., Kozak, S., Kabat, D., & Geib, R. W. (1992). Mutations in the env gene of friend spleen focus-forming virus overcome Fv-2r-mediated resistance to friend virus-induced erythroleukemia. Journal of Virology, 66(6), 3652-3660.

Mutations in the env gene of friend spleen focus-forming virus overcome Fv-2r-mediated resistance to friend virus-induced erythroleukemia. / Majumdar, Manas K.; Cho, Chung Lung; Fox, Mary T.; Eckner, Kristine L.; Kozak, Sue; Kabat, David; Geib, Roy W.

In: Journal of Virology, Vol. 66, No. 6, 06.1992, p. 3652-3660.

Research output: Contribution to journalArticle

Majumdar, MK, Cho, CL, Fox, MT, Eckner, KL, Kozak, S, Kabat, D & Geib, RW 1992, 'Mutations in the env gene of friend spleen focus-forming virus overcome Fv-2r-mediated resistance to friend virus-induced erythroleukemia', Journal of Virology, vol. 66, no. 6, pp. 3652-3660.
Majumdar, Manas K. ; Cho, Chung Lung ; Fox, Mary T. ; Eckner, Kristine L. ; Kozak, Sue ; Kabat, David ; Geib, Roy W. / Mutations in the env gene of friend spleen focus-forming virus overcome Fv-2r-mediated resistance to friend virus-induced erythroleukemia. In: Journal of Virology. 1992 ; Vol. 66, No. 6. pp. 3652-3660.
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abstract = "Although Fv-2r homozygous mice are resistant to leukemias induced either by an erythropoietin-encoding virus or by wild-type Friend virus (FV) (M. E. Hoatlin, S. L. Kozak, F. Lilly, A. Chakraborti, C. A. Kozak, and D. Kabat, Proc. Natl. Acad. Sci. USA 87:9985-9989, 1990), they are susceptible to some variants of FV (R. A. Steeves, E. A. Mirand, A. Bulba, and P. J. Trudel, Int. J. Cancer 5:349-356, 1970; R. W. Geib, M. B. Seaward, M. L. Stevens, C.-L. Cho, and M. Majumdar, Virus Res. 14:161-174, 1989). To localize the virus gene involved in influencing the host range, we cloned and sequenced the env gene of the BB6 variant of FV (Steeves et al., Int. J. Cancer 5:349-356, 1970). In comparison with the wild-type env gene, the BB6 variant contains a 159-bp deletion that eliminates the membrane-proximal portion of the extracellular domain and 58 point mutations resulting in 13 amino acid changes. Substitution of the variant env gene for the wild-type env gene resulted in a recombinant virus that produced a Friend virus-like disease in Fv-2r homozygotes. Our results identify the spleen focus-forming virus env gene as the viral gene involved in this virus-host interaction. Additionally, they suggest that the product of the Fv-2r gene modifies the interaction between the spleen focus-forming virus envelope protein and the erythropoietin receptor.",
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