Mutations in the env gene of friend spleen focus-forming virus overcome Fv-2r-mediated resistance to friend virus-induced erythroleukemia

Manas K. Majumdar, Chung Lung Cho, Mary T. Fox, Kristine L. Eckner, Sue Kozak, David Kabat, Roy W. Geib

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Although Fv-2r homozygous mice are resistant to leukemias induced either by an erythropoietin-encoding virus or by wild-type Friend virus (FV) (M. E. Hoatlin, S. L. Kozak, F. Lilly, A. Chakraborti, C. A. Kozak, and D. Kabat, Proc. Natl. Acad. Sci. USA 87:9985-9989, 1990), they are susceptible to some variants of FV (R. A. Steeves, E. A. Mirand, A. Bulba, and P. J. Trudel, Int. J. Cancer 5:349-356, 1970; R. W. Geib, M. B. Seaward, M. L. Stevens, C.-L. Cho, and M. Majumdar, Virus Res. 14:161-174, 1989). To localize the virus gene involved in influencing the host range, we cloned and sequenced the env gene of the BB6 variant of FV (Steeves et al., Int. J. Cancer 5:349-356, 1970). In comparison with the wild-type env gene, the BB6 variant contains a 159-bp deletion that eliminates the membrane-proximal portion of the extracellular domain and 58 point mutations resulting in 13 amino acid changes. Substitution of the variant env gene for the wild-type env gene resulted in a recombinant virus that produced a Friend virus-like disease in Fv-2r homozygotes. Our results identify the spleen focus-forming virus env gene as the viral gene involved in this virus-host interaction. Additionally, they suggest that the product of the Fv-2r gene modifies the interaction between the spleen focus-forming virus envelope protein and the erythropoietin receptor.

Original languageEnglish (US)
Pages (from-to)3652-3660
Number of pages9
JournalJournal of Virology
Volume66
Issue number6
Publication statusPublished - Jun 1992
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Immunology

Cite this

Majumdar, M. K., Cho, C. L., Fox, M. T., Eckner, K. L., Kozak, S., Kabat, D., & Geib, R. W. (1992). Mutations in the env gene of friend spleen focus-forming virus overcome Fv-2r-mediated resistance to friend virus-induced erythroleukemia. Journal of Virology, 66(6), 3652-3660.