Mutations in pts cause catabolite-resistant sporulation and altered regulation of spoOH in Bacillus subtilis

D. Frisby, P. Zuber

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Abstract

A mutation in Bacillus subtilis, ggr-31, that relieves glucose-glutamine- dependent control of a spoVG-lacZ translational fusion was isolated and was subsequently found to confer a pleiotropic phenotype. Mutants cultured in glucose- and glutamine-rich media exhibited a Crs- (catabolite-resistant sporulation) phenotype; enhanced expression of the spo0H gene, encoding σ(H), as evidenced by immunoblot analysis with anti-σ(H) antiserum; and derepression of srfA, an operon involved in surfactin biosynthesis and competence development. In addition, ggr-31 mutants exhibited a significant increase in generation time when they were cultured in minimal glucose medium. The mutant phenotype was restored to the wild type by Campbell integration of a plasmid containing part of the ptsG (encoding the enzyme II/III glucose permease) gene, indicating that the mutation probably resides within ptsG and adversely affects glucose uptake. A deletion mutation within ptsI exhibited a phenotype similar to that of ggr-31.

Original languageEnglish (US)
Pages (from-to)2587-2595
Number of pages9
JournalJournal of bacteriology
Volume176
Issue number9
DOIs
StatePublished - Jan 1 1994

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ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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