Mutations associated with viral sequences isolated from mice persistently infected with MHV-JHM

J. O. Fleming; C. Adami; J. Pooley; J. Glomb; E. Stecker; F. Fazal; S. C. Baker

doi:10.1007/978-1-4615-1899-0_94

Mutations associated with viral sequences isolated from mice persistently infected with MHV-JHM

J. O. Fleming, C. Adami, J. Pooley, J. Glomb, E. Stecker, F. Fazal, S. C. Baker

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Mouse hepatitis virus JHM (JHMV or MHV-4) induces subacute and chronic demyelination in rodents and has been studied as a model of human demyelinating diseases, such as multiple sclerosis. However, despite intensive investigation, the state of JHMV during chronic disease is poorly understood. Using reverse transcription-polymerase chain reaction amplification (RT-PCR) to 'rescue' viral RNA, we have found that JHMV- specific sequences persist for at least 787 days after intracerebral inoculation of experimental mice. Analysis of persisting vital RNA reveals that it is extensively mutated, and we hypothesize that the mutations observed reflect adaptation of the vital quasispecies to low-level intracellular replication during chronic disease.

Original language	English (US)
Pages (from-to)	591-595
Number of pages	5
Journal	Advances in experimental medicine and biology
Volume	380
DOIs	https://doi.org/10.1007/978-1-4615-1899-0_94
State	Published - 1995
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "Mutations associated with viral sequences isolated from mice persistently infected with MHV-JHM",

abstract = "Mouse hepatitis virus JHM (JHMV or MHV-4) induces subacute and chronic demyelination in rodents and has been studied as a model of human demyelinating diseases, such as multiple sclerosis. However, despite intensive investigation, the state of JHMV during chronic disease is poorly understood. Using reverse transcription-polymerase chain reaction amplification (RT-PCR) to 'rescue' viral RNA, we have found that JHMV- specific sequences persist for at least 787 days after intracerebral inoculation of experimental mice. Analysis of persisting vital RNA reveals that it is extensively mutated, and we hypothesize that the mutations observed reflect adaptation of the vital quasispecies to low-level intracellular replication during chronic disease.",

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T1 - Mutations associated with viral sequences isolated from mice persistently infected with MHV-JHM

AU - Fleming, J. O.

AU - Adami, C.

AU - Pooley, J.

AU - Glomb, J.

AU - Stecker, E.

AU - Fazal, F.

AU - Baker, S. C.

PY - 1995

Y1 - 1995

N2 - Mouse hepatitis virus JHM (JHMV or MHV-4) induces subacute and chronic demyelination in rodents and has been studied as a model of human demyelinating diseases, such as multiple sclerosis. However, despite intensive investigation, the state of JHMV during chronic disease is poorly understood. Using reverse transcription-polymerase chain reaction amplification (RT-PCR) to 'rescue' viral RNA, we have found that JHMV- specific sequences persist for at least 787 days after intracerebral inoculation of experimental mice. Analysis of persisting vital RNA reveals that it is extensively mutated, and we hypothesize that the mutations observed reflect adaptation of the vital quasispecies to low-level intracellular replication during chronic disease.

AB - Mouse hepatitis virus JHM (JHMV or MHV-4) induces subacute and chronic demyelination in rodents and has been studied as a model of human demyelinating diseases, such as multiple sclerosis. However, despite intensive investigation, the state of JHMV during chronic disease is poorly understood. Using reverse transcription-polymerase chain reaction amplification (RT-PCR) to 'rescue' viral RNA, we have found that JHMV- specific sequences persist for at least 787 days after intracerebral inoculation of experimental mice. Analysis of persisting vital RNA reveals that it is extensively mutated, and we hypothesize that the mutations observed reflect adaptation of the vital quasispecies to low-level intracellular replication during chronic disease.

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Mutations associated with viral sequences isolated from mice persistently infected with MHV-JHM

Abstract

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