Mutational analysis of the Bacillus subtilis RNA polymerase α C-terminal domain supports the interference model of Spx-dependent repression

Ying Zhang, Shunji Nakano, Soon Yong Choi, Peter Zuber

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The Spx protein of Bacillus subtilis exerts both positive and negative transcriptional control in response to oxidative stress by interacting with the C-terminal domain of the RNA polymerase (RNAP) alpha subunit (αCTD). Thus, transcription of the srf operon at the onset of competence development, which requires the ComA response regulator of the ComPA signal transduction system, is repressed by Spx-αCTD interaction. Previous genetic and structural analyses have determined that an Spx-binding surface resides in and around the α-region of αCTD. Alanine-scanning mutagenesis of B. subtilis αCTD uncovered residue positions required for Spx function and ComA-dependent srf transcriptional activation. Analysis of srf-lacZ fusion expression, DNase I footprinting, and solid-phase promoter retention experiments indicate that Spx interferes with ComA-aCTD interaction and that residues Y263, C265, and K267 of the α1-region lie within overlapping ComA- and Spx-binding sites for αCTD interaction. Evidence is also presented that oxidized Spx, while enhancing interference of activator-RNAP interaction, is not essential for negative control.

Original languageEnglish (US)
Pages (from-to)4300-4311
Number of pages12
JournalJournal of bacteriology
Volume188
Issue number12
DOIs
StatePublished - Jun 1 2006

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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