Mutational analysis of Ca2+-independent autophosphorylation of calcium/calmodulin-dependent protein kinase II

Sucheta Mukherji, Thomas Soderling

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    Previous studies with synthetic peptides indicate that residues 290-309, corresponding to the calmodulin (CaM)-binding domain of Ca2+/CaM-dependent protein kinase II interact with the catalytic core of the enzyme as a pseudosubstrate (Colbran, R. J., Smith, M. K., Schworer, C. M., Fong, Y. L., and Soderling, T. R. (1989) J. Biol. Chem. 264, 4800-4804). In the present study, we attempted to locate the pseudosubstrate motif by generation or removal of potential substrate recognition sequences (RXXS/T) at selected positions using site-directed mutagenesis. Based on previous results, Arg297, Thr305/306, and Ser314 were selected as key residues. Single mutations such as N294S, K300S, A302R, A309R, and R311A were expressed, purified, and characterized. Several of the mutants exhibited decreased binding of and activation by CaM, not surprising since the mutations were within the CaM-binding domain. None of the mutants exhibited enhanced Ca2+-independent kinase activity toward exogenous substrate, but the K300S and N294S mutants showed a significant enhancement in the rate and stoichiometry of 32P incorporation during Ca2+-independent autophosphorylation. Using two-dimensional peptide mapping and phosphoamino acid analyses, enhanced phosphorylation of the introduced Ser residue was demonstrated in the K300S mutant but not in the N294S mutant. This specific Ca2+-independent autophosphorylation of Ser300 is consistent with the hypothesis that Arg297 may occupy the P (-3) position in a pseudosubstrate autoinhibitory interaction with the catalytic core in the nonactivated state of the kinase.

    Original languageEnglish (US)
    Pages (from-to)14062-14067
    Number of pages6
    JournalJournal of Biological Chemistry
    Volume270
    Issue number23
    StatePublished - Jun 9 1995

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    Calcium-Calmodulin-Dependent Protein Kinase Type 2
    Calcium-Calmodulin-Dependent Protein Kinases
    Calmodulin
    Catalytic Domain
    Phosphotransferases
    Phosphoamino Acids
    Mutagenesis
    Peptides
    Mutation
    Phosphorylation
    Peptide Mapping
    Substrates
    Site-Directed Mutagenesis
    Stoichiometry
    Chemical activation
    Enzymes

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Mutational analysis of Ca2+-independent autophosphorylation of calcium/calmodulin-dependent protein kinase II. / Mukherji, Sucheta; Soderling, Thomas.

    In: Journal of Biological Chemistry, Vol. 270, No. 23, 09.06.1995, p. 14062-14067.

    Research output: Contribution to journalArticle

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