Mutation frequency is reduced in the cerebellum of Big Blue® mice overexpressing a human wild type SOD1 gene

Makoto Kunishige, Kathleen A. Hill, Amanda M. Riemer, Kelly D. Farwell, Asanga Halangoda, Ernst Heinmöller, Stephen R. Moore, Dianna M. Turner, Steve S. Sommer

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Amyotrophic lateral sclerosis (ALS) is a progressive paralytic disorder caused by motor neuron degeneration. A similar disease phenotype is observed in mice overexpressing a mutant human hSOD1 gene (G93A, 1Gurd1). Mice transgenic for lacI (Big Blue®) and human mutant (1Gurd1, Mut hSOD1) or wild type (2Gur, Wt hSOD1) SOD1 genes were used to examine spontaneous mutation, oxidative DNA damage, and neurodegeneration in vivo. The frequency and pattern of spontaneous mutation were determined for forebrain (90% glia), cerebellum (90% neurons) and thymus from 5-month-old male mice. Mutation frequency is not elevated significantly and mutation pattern is unaltered in Mut hSOD1 mice compared to control mice. Mutation frequency is reduced significantly in the cerebellum of Wt hSOD1 mice (1.6 × 10-5; P = 0.0093; Fisher's Exact Test) compared to mice without a human transgene (2.7 × 10-5). Mutation pattern is unaltered. This first report of an endogenous factor that can reduce in vivo, the frequency of spontaneous mutation suggests potential strategies for lowering mutagenesis related to aging, neurodegeneration, and carcinogenesis.

Original languageEnglish (US)
Pages (from-to)139-149
Number of pages11
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume473
Issue number2
DOIs
StatePublished - Feb 20 2001

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Keywords

  • Amyotrophic lateral sclerosis
  • LacI
  • Neurodegeneration
  • Oxidative damage
  • Superoxide dismutase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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