Muscarinic receptor loss and preservation of presynaptic cholinergic terminals in hippocampal sclerosis

P. B. Pennell, D. E. Burdette, Donald (Don) Ross, T. R. Henry, R. L. Albin, J. C. Sackellares, K. A. Frey

Research output: Contribution to journalArticle

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Abstract

Purpose: Prior single-photon emission tomography studies showed losses of muscarinic acetylcholine receptor (MAChR) binding in patients with refractory mesial temporal lobe epilepsy. Experimental animal studies demonstrated transient losses of MAChR due to electrically induced seizures originating in the amygdala. However, the relations between cholinergic synaptic markers, seizures, and underlying neuropathology in human temporal lobe epilepsy are unknown. We tested the hypotheses that human brain MAChR changes are attributable to hippocampal sclerosis (HS), and that HS resembles axon-sparing lesions in experimental animal models. Methods: We measured MAChR binding-site density, an intrinsic neuronal marker, within the hippocampal formation (HF) in anterior temporal lobectomy specimens from 10 patients with HS and in 10 autopsy controls. Binding-site density of the presynaptic vesicular acetylcholine transporter (VAChT) was measured as a marker of extrinsic cholinergic afferent integrity. MAChR and VAChT results were compared with neuronal cell counts to assess their relations to local neuronal losses. Results: Reduced MAChR binding-site density was demonstrated throughout the HF in the epilepsy specimens compared with autopsy controls and correlated in severity with reductions in cell counts in several HF regions. In contrast to MAChR, VAChT binding-site density was unchanged in the epilepsy specimens compared with autopsy controls. Conclusions: Reduction in MAChR binding in HS is attributable to intrinsic neuronal losses. Sparing of afferent septal cholinergic terminals is consistent with the hypothesis that an excitotoxic mechanism may contribute to the development of HS and refractory partial epilepsy in humans.

Original languageEnglish (US)
Pages (from-to)38-46
Number of pages9
JournalEpilepsia
Volume40
Issue number1
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Presynaptic Terminals
Sclerosis
Muscarinic Receptors
Cholinergic Agents
Vesicular Acetylcholine Transport Proteins
Binding Sites
Autopsy
Hippocampus
Temporal Lobe Epilepsy
Epilepsy
Seizures
Cell Count
Anterior Temporal Lobectomy
Partial Epilepsy
Amygdala
Photons
Axons
Animal Models
Tomography
Brain

Keywords

  • Excitotoxicity
  • Mesial temporal epilepsy
  • Muscarinic

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Pennell, P. B., Burdette, D. E., Ross, D. D., Henry, T. R., Albin, R. L., Sackellares, J. C., & Frey, K. A. (1999). Muscarinic receptor loss and preservation of presynaptic cholinergic terminals in hippocampal sclerosis. Epilepsia, 40(1), 38-46. https://doi.org/10.1111/j.1528-1157.1999.tb01986.x

Muscarinic receptor loss and preservation of presynaptic cholinergic terminals in hippocampal sclerosis. / Pennell, P. B.; Burdette, D. E.; Ross, Donald (Don); Henry, T. R.; Albin, R. L.; Sackellares, J. C.; Frey, K. A.

In: Epilepsia, Vol. 40, No. 1, 1999, p. 38-46.

Research output: Contribution to journalArticle

Pennell, PB, Burdette, DE, Ross, DD, Henry, TR, Albin, RL, Sackellares, JC & Frey, KA 1999, 'Muscarinic receptor loss and preservation of presynaptic cholinergic terminals in hippocampal sclerosis', Epilepsia, vol. 40, no. 1, pp. 38-46. https://doi.org/10.1111/j.1528-1157.1999.tb01986.x
Pennell, P. B. ; Burdette, D. E. ; Ross, Donald (Don) ; Henry, T. R. ; Albin, R. L. ; Sackellares, J. C. ; Frey, K. A. / Muscarinic receptor loss and preservation of presynaptic cholinergic terminals in hippocampal sclerosis. In: Epilepsia. 1999 ; Vol. 40, No. 1. pp. 38-46.
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