TY - JOUR
T1 - Murine monoclonal anti-myelin basic protein (MBP) antibodies inhibit proliferation and cytotoxicity of MBP-specific human T cell clones
AU - Jingwu, Zhang
AU - Vandenbark, Arthur A.
AU - Jacobs, Marie Paule
AU - Offner, Halina
AU - Raus, Jef C.M.
PY - 1989/9
Y1 - 1989/9
N2 - Myelin basic protein (MBP)-specific T cell clones, isolated from two patients with multiple sclerosis, expressed the CD4+ phenotype and induced MBP-dependent cytolysis of autologous Epstein-Barr virus (EBV)-transformed B cells. The proliferation and cytolytic activity of the T cell clones were inhibited by four of a panel of five murine monoclonal anti-MBP antibodies in a dose-dependent manner. An isotype-matched antibody with a irrelevant specificity did not have such an effect. These MBP-specific monoclonal antibodies did not block phytohemagglutinin-induced T cell proliferation or allospecific cytotoxicity. These results suggest that some antibodies directed at the autoantigen MBP may play a regulatory role in T cell activation, rather than a pathogenic role, for which there is currently little supporting evidence.
AB - Myelin basic protein (MBP)-specific T cell clones, isolated from two patients with multiple sclerosis, expressed the CD4+ phenotype and induced MBP-dependent cytolysis of autologous Epstein-Barr virus (EBV)-transformed B cells. The proliferation and cytolytic activity of the T cell clones were inhibited by four of a panel of five murine monoclonal anti-MBP antibodies in a dose-dependent manner. An isotype-matched antibody with a irrelevant specificity did not have such an effect. These MBP-specific monoclonal antibodies did not block phytohemagglutinin-induced T cell proliferation or allospecific cytotoxicity. These results suggest that some antibodies directed at the autoantigen MBP may play a regulatory role in T cell activation, rather than a pathogenic role, for which there is currently little supporting evidence.
KW - Monoclonal antibody, myelin basic protein-specific
KW - Multiple sclerosis
KW - Myelin basic protein
KW - T cell clone
KW - myelin basic protein-specific
UR - http://www.scopus.com/inward/record.url?scp=0024447017&partnerID=8YFLogxK
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U2 - 10.1016/0165-5728(89)90102-1
DO - 10.1016/0165-5728(89)90102-1
M3 - Article
C2 - 2478579
AN - SCOPUS:0024447017
VL - 24
SP - 87
EP - 94
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -