TY - JOUR
T1 - Murine monoclonal anti-myelin basic protein (MBP) antibodies inhibit proliferation and cytotoxicity of MBP-specific human T cell clones
AU - Jingwu, Zhang
AU - Vandenbark, Arthur A.
AU - Jacobs, Marie Paule
AU - Offner, Halina
AU - Raus, Jef C.M.
N1 - Funding Information:
The authors wish to thank Mr. J. Lambrechts for FACS analysis, Dr. H. Heyligen and Mr. Eugene Bosmans for helpful discussions and Miss Paula Hendrikx for manuscript preparation. This study was supported in part by WOMS Belgium, the Veterans Administration and by NIH Grant NS23221, U.S.A.
PY - 1989/9
Y1 - 1989/9
N2 - Myelin basic protein (MBP)-specific T cell clones, isolated from two patients with multiple sclerosis, expressed the CD4+ phenotype and induced MBP-dependent cytolysis of autologous Epstein-Barr virus (EBV)-transformed B cells. The proliferation and cytolytic activity of the T cell clones were inhibited by four of a panel of five murine monoclonal anti-MBP antibodies in a dose-dependent manner. An isotype-matched antibody with a irrelevant specificity did not have such an effect. These MBP-specific monoclonal antibodies did not block phytohemagglutinin-induced T cell proliferation or allospecific cytotoxicity. These results suggest that some antibodies directed at the autoantigen MBP may play a regulatory role in T cell activation, rather than a pathogenic role, for which there is currently little supporting evidence.
AB - Myelin basic protein (MBP)-specific T cell clones, isolated from two patients with multiple sclerosis, expressed the CD4+ phenotype and induced MBP-dependent cytolysis of autologous Epstein-Barr virus (EBV)-transformed B cells. The proliferation and cytolytic activity of the T cell clones were inhibited by four of a panel of five murine monoclonal anti-MBP antibodies in a dose-dependent manner. An isotype-matched antibody with a irrelevant specificity did not have such an effect. These MBP-specific monoclonal antibodies did not block phytohemagglutinin-induced T cell proliferation or allospecific cytotoxicity. These results suggest that some antibodies directed at the autoantigen MBP may play a regulatory role in T cell activation, rather than a pathogenic role, for which there is currently little supporting evidence.
KW - Monoclonal antibody, myelin basic protein-specific
KW - Multiple sclerosis
KW - Myelin basic protein
KW - T cell clone
KW - myelin basic protein-specific
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U2 - 10.1016/0165-5728(89)90102-1
DO - 10.1016/0165-5728(89)90102-1
M3 - Article
C2 - 2478579
AN - SCOPUS:0024447017
VL - 24
SP - 87
EP - 94
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -